Fiziol. rast. genet. 2021, vol. 53, no. 1, 63-73, doi:

Effect of tebuconazole on the use of reserve substances deposited in the seed of Vicia faba L. at the hete-rotrophic phase of development under conditions of photo- and scotomorphogenesis

Kuryata V.G., Kuts B.A., Poprotska I.V.

  • Mykhailo Kotsyubynsky Vinnytsia State Pedagogical University 32 Ostrozhskoho St., Vinnytsia, 21001, Ukraine

The combination of external (light/dark) factor and the action of the gibberellin synthesis inhibitor tebuconazole during seed germination significantly changed the pattern of source-sink relationships in horse beans seedlings. Under the action of the tebuconazole the epicotyl, root, and seedling length in general were reduced significantly, both at light and dark. Similarly, the dry matter mass of the seedling organs was decreased. Seed reserve substances were used more intensively under conditions of scotomorphogenesis, as evidenced by the minimum cotyledons dry matter mass in this variant and higher utilization rates of reserve substances for root and epicotyl formation during germination. The retardant application led to a slowdown in the outflow of seed reserves for the formation of epicotyl and root. Under conditions of scotomorphogenesis, the rate of reserve seed starch use was higher. The gibberellin biosynthesis inhibitor tebuconazole did not affect the rate of cotyledon starch hydrolysis, indicating an internal sufficient supply by reserved forms of gibberellins. The higher sugar content in the cotyledons of scotomorphic plants, both in the control and under the action of tebuconazole, is explained by the slowing of the outflow for the needs of organogenesis — the formation of root and epicotyl structures. Quantitative changes of the nitrogen content in the cotyledons of scotomorphic and photormorphic plants during germination were much smaller than the changes of the starch content. It was no significant effect of tebuconazole on the remobilization of nitrogen-containing compounds and mineral nutrients — phosphorus and potassium deposited in the seed, on the needs of organogenesis.

Keywords: Vicia faba L., morphogenesis, source-sink system, seed germination, light, retardants

Fiziol. rast. genet.
2021, vol. 53, no. 1, 63-73

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1. Kiriziy, D.A., Stasyk, O.O., Pryadkina, G.A. & Shadchyna, T.M. (2014). Photosynthesis. CO2 assimilation and mechanisms of its regulation. Kyiv: Logos [in Russian].

2. Bonelli, L.E., Monzon, J. P., Cerrudo, A., Rizzalli, R. H. & Andrade, F. H. (2016). Maize grain yield components and source-sink relationship as affected by the delay in sowing date. Field Crops Research, 198, pp. 215-225.

3. Kuryata, V.G. & Golunova, LA. (2018). Peculiarities of the formation and functioning of soybean-rhizobial complexes and the productivity of soybean culture under the influence of retardant of paclobutrazol. Ukr. J. Ecol., 8(3), pp. 98-105.

4. Yu, S.M., Lo, S.F. & Ho, T.D. (2015). Source-sink communication: regulated by hormone, nutrient, and stress cross-signaling. Trends in Plant Science, 20(12), pp. 844-857.

5. Kuryata, V.G. & Kravets, O.O. (2018). Features of morphogenesis, accumulation and redistribution of assimilate and nitrogen containing compounds in tomatoes under retardants treatment. Ukr. J. Ecol., 8(1), pp. 356-362.

6. Kuryata, V.G. & Khodanitska, O.O. (2018). Features of an atomical structure, formation and functioning of leaf apparatus and productivity of linseed under chlormequat chloride treatment. Ukr. J. Ecol., 8(1), pp. 918-926.

7. Kuryata, V.G., Rogach, V.V., Buina, O.I. & Kushnir, O.V. (2017). Impact of gibberelic acid and tebuconazole on formation of the leaf system and functioning of donor-acceptor plant system of solanaceae vegetable crops. Regulatory Mechanisms in Biosystems, 8(2), pp. 162-168.

8. Matysiak, K. & Kaczmarek, S. (2013). Effect of chlorocholine chloride and triazoles - tebuconazole and flusilazole on winter oilseed rape (Brassica napus var. oleifera L.) in response to the application term and sowing density. Journal of plant protection research, 53(1), pp. 79-88.

9. Savage, J.A., Haines, D.F., & Holbrook, N.M. (2015) The making of giant pumpkins: how selective breeding changed the phloem of Cucurbita maxima from source to sink. Plant Cell Environ., 38(8), pp. 1543-1554.

10. Sugiura, D., Sawakami, K., Kojim, M., Sakakibara, H., Terashima, I. & Tateno, M. (2015). Roles of gibberellins and cytokinins in regulation of morphological and physiological traits in Polygonum cuspidatum responding to light and nitrogen availabilities. Funct. Plant Biol., 42(4), pp. 390-397.

11. Jiang, L. Li, S. (2015). Signaling Cross Talk Under the Control of Plant Photoreceptors. Bjorn L. (Eds.) Photobiology. Springer, New York, pp. 177-187.

12. Hornitschek, P., Kohnen, M.V., Lorrain, S., Rougemont, J., Ljung, K., Lopez-Vidriero, I., Franco-Zorrilla, J.M., Solano, R., Trevisan, M., Pradervand, S., Xenarios, I. & Fank­hauser, C. (2012). Phytochrome interacting factors 4 and 5 control seedling growth in changing light conditions by directly controlling auxin signaling. Plant J., 71(5), pp. 699-711.

13. Wu, S.H. (2014). Gene expression regulation in photomorphogenesis from the perspective of the central dogma. Annu. Rev. Plant Biol., 65, pp. 311-333.

14. Franklin, K.A. (2016). Photomorphogenesis: plants feel blue in the shade. Curr. Biol., 26(24), pp. 1275-1276.

15. Josse, E.M. &Halliday, K.J. (2008). Skotomorphogenesis: The Dark Side of Light Signalling. Current Biology, 18(24), pp. 1144-1146.

16. Kutschera, U. & Briggs, W.R. (2003). Seedling development in buckwheat and the discovery of the photomorphogenic shade-avoidance response. Plant Biol. (Stuttg), 15(6), pp. 931-940.

17. Nakaminami, K., Sawada, Y., Suzuki, M., Kenmoku, H., Kawaide, H., Mitsuhashi, W., Sassa, T., Inoue, Y., Kamiya, Y. & Toyomasu, T. (2003). Deactivation of gibberellin by 2-oxidation during germination of photoblastic lettuce seeds. Biosci Biotechnol Biochem., 67(7), pp. 1551-1558.

18. De Wit. M. & Pierik. R. (2016). Photomorphogenesis and Photoreceptors. Canopy Photosynthesis: From Basics to Applications., 42, pp. 171-186.

19. Hedden, P. & Thomas, S.G. (2016). Annual Plant Reviews. Vol. 49. The Gibberellins. John Wiley & Sons, 472 p.

20. Folta, K.M., Pontin, M.A. & Karlin-Neumann, G. (2003). Genomic and physiological studies of early cryptochrome 1 action demonstrate roles for auxin and gibberellin in the control of hypocotyl growth by blue light. Plant J., 36(2), pp. 203-214.

21. Kusnetsov, V.V., Doroshenko, A.S. & Kudryakova, N.V. (2020). Role of phytohormones and light in de-etiolation. Russian Journal of Plant Physiology, 67, pp. 971-984.

22. Rizza, A. & Jones, A.M. (2019). The makings of a gradient: spatiotemporal distribution of gibberellins in plant development. Current Opinion in Plant Biology, 47, pp. 9-15.

23. Poprotska, I.V. & Kuryata, V.G. (2017). Features of gas exchange and use of reserve substances in pumpkin seedlings in conditions of skoto- and photomorphogenesis under the influence of gibberellin and chlormequat-chloride. Regulatory Mechanisms in Biosystems, 8(1), pp. 71-76.

24. Poprotska, I.V. (2016). Regulation of sourse-sink relations in plants in the assimilates depot-growth system during germination. Vinnytsia: TOV "Nilan-LTD" [in Ukrainian].

25. Poprotska, I., Kuryata, V., Khodanitska, O., Polyvanyi, S., Golunova, L. & Prysedsky, Yu. (2019). Effect of gibberellin and retardants on the germination of seeds with different types of reserve substances under the conditions of skoto- and photomorphogenesis. Biologija, 65(4), pp. 296-307.

26. Kuryata, V.G. & Poprotska, I.V. (2019). Physiological and biochemical basics of application of retardants in plant growing. Vinnitsa: "Tvory" [in Ukrainian].

27. AOAC. (2010). Official methods of analysis of association of analytical chemist international 18th ed. Rev. 3. Asso of Analytical Chemist. Gaithersburg, Maryland, USA.

28. Rademacher, W. (2016). Chemical regulators of gibberellin status and their application in plant production. Annu. Plant Rev., 49, pp. 359-403.

29. Kuryata, V., Kuts, B. & Prysedsky, Yu (2020). Effect of gibberellin on the use of reserve substances deposited in Vicia faba L. seeds at the phase of heterotrophic development under the conditions of photo- and skotomorphogenesis. Biologija, 66 (3), pp. 159-167.