Fiziol. rast. genet. 2021, vol. 53, no. 5, 435-443, doi:

Structural and functional dependence of PSII activity under oxidative stress

Jafarova J.1, Ganieva R.1, Mammadova L.2, Agalarov R.2, Gasanov R.2

  1. Institute of Botany, National Academy of Sciences of Azerbaijan Baku, AZ 1073, Azerbaijan
  2. Biotechnology Laboratory, Department of Biophysics and Molecular Biology Baku State University, Baku AZ1021, Azerbaijan

The action of methyl viologen (MV) on pumpkin leaves (Cucurbita pepo L.) increased the content of reactive oxygen species (ROS) that caused disruption of the PSII electron transport chain. After the treatment by MV the seedlings were treated by protein synthesis inhibitor — chloramphenicol (CAP). The millisecond delay fluorescence of chlorophyll (Chl) a induction curves analysis (msec-DF Chl a) is shown the change of intensity of phase of msec-DF Chl a, possible in result of suppression D1 protein de novo formation. The changes of proteins profiles especially of PSII polypeptides content in chloroplasts in dependence on action of methyl viologen and chloramphenicol were observed. The high correlation between changes of PSII polypeptides and changing of stationary phase of msec-DF Chl a were shown.

Keywords: Cucurbita pepo L., oxidative damage, millisecond delay fluorescence of Chl a, reactive oxygen species, methylviologen

Fiziol. rast. genet.
2021, vol. 53, no. 5, 435-443

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