Fiziol. rast. genet. 2020, vol. 52, no. 1, 46-63, doi:

Effect of foliar treatment with microelement complex, obtained by nanotechnology, on the photosynthetic activity of winter wheat plants under different moisture

Stasik O.O.1, Pryadkina G.O.1, Kiriziy D.A.1, Sytnik S.K.1, Kapitanska O.S.2, Mikhno A.I.1, Makharinska N.M.1

  1. Institute of Plant Physiology and Genetics, National Academy of Sciences of Ukraine 31/17 Vasylkivska St., Kyiv, 03022, Ukraine
  2. Research and Production Company “Kvadrat” sec. 7, 41/2, Gagarin Ave., Kharkiv, 61001, Ukraine

The effect of foliar treatment of winter wheat plants with the microelement complex Avatar-1 created using nanotechnologies, containing chelated with natural carboxylic acids magnesium, copper, iron, zinc, manganese, molybdenum and cobalt, on the flag leaf photosynthetic activity under different soil moisture conditions was studied. The studies were carried out in a pot experiment on two varieties of winter bread wheat (Triticum aestivum L.), a high-yielding Astarta variety characterized by a long-lasting activity of the photosynthetic apparatus during the grain filling period (stay-green phenotype), and Natalka variety with a high grain protein content. At the heading stage (BBCH 59), the experimental plants were sprayed with a microelement complex. Plants treated with tap water served as a control. Six days after treatment at the beginning of the anthesis stage (BBCH 61), half of the experimental and control plants were exposed to drought (7 days at soil moisture 30 % of field capacity (FC)), the another half remained at an optimum moisture supply of 70 % FC. It was found that drought significantly reduced the net assimilation rate compared with normal irrigation. However, the decrease in photosynthetic activity in plants treated with the microelement complex was less (36 and 33 %) comparing to untreated plants (46 and 52 %) in varieties Astarta and Natalka, respectively. Under moisture deficiency, the photosynthetic rate in plants treated with a microelement complex was higher than in untreated plants – by 22 % for Astarta and 34 % for Natalka. The photorespiration rate in flag leaf, in contrast to photosynthesis, increased significantly under drought conditions. In untreated Astarta variety plants the increase was greater (82 %) compared with plants treated with the microelement complex (39 %), but, on the contrary, in Natalka variety it was less in treated plants (44 %) compared with untreated (96 %). Treatment with a microelement complex increased the PS II photochemical activity in the flag leaf both under optimal and limited moisture supply, mitigating drought damaging effect. The decrease in the PS II maximum quantum efficiency was about 2 % in the treated plants of both varieties and 5 and 12 %, respectively, in the control plants of Astarta and Natalka varieties. It was concluded that foliar treatment of winter wheat plants with a microelement complex, obtained by nanotechnology, significantly increases the photosynthetic apparatus resistance to soil drought, although it does not cause significant changes in the CO2 assimilation rate under optimal moisture supply. Maintaining high level of CO2 assimilation and PS II photochemical activity under drought conditions due to the treatment with microelement complex contributed to an increase in the grain productivity of plants. The positive effect of microfertilizer on grain productivity was more pronounced in the less resistant to drought variety.

Keywords: Triticum aestivum L., CO2 gas exchange, PS II photochemical activity, microelements chelated with carboxylic acids, grain productivity

Fiziol. rast. genet.
2020, vol. 52, no. 1, 46-63

Full text and supplemented materials

Free full text: PDF  


1. Lesk, C., Rowhani, P. & Ramankutty, N. (2016). Influence of extreme weather disasters on global crop production. Nature, 529, No. 7584, pp. 84-87.

2. IPCC: Summary for policymakers. In: Climate Change 2014: Impacts, Adaptation, and Vulnerability. Pt A: Global and Sectoral Aspects. Contribution of Working Group II to the Fifth Assessment Report of the Intergovernmental Panel on Climate Change. Field, C.B., Barros, V.R., Dokken, D.J., Mach, K.J., Mastrandrea, M.D., Bilir, T.E., Chatterjee, M., Ebi, K.L., Estrada, Y.O., Genova, R.C., Girma, B., Kissel, E.S., Levy, A.N., MacCracken, S., Mastrandrea, P.R. & White, L.L. (Eds.) (pp. 1-32), New York USA: Cambridge University Press, Cambridge, United Kingdom and New York. 2014.

3. Reynolds, M.P., Quilligan, E., Aggarwal, P.K., Kailash, C., Bansal, K.C., Cavalieri, A.J., Chapman, S.C., Chapotin, S.M., Datta, S.K., Duveiller, E., Gill, K.S., Jagadish, K.S.V., Joshi, A.K., Koehler, A.-K., Kosina, P., Krishnan, S., Lafitte, R., Mahala, R.S., Muthurajan, R., Paterson, A.H., Prasanna, B.M., Rakshit, S., Rosegrant, M.W., Sharma, I., Singh, R.P., Sivasankar, S., Vadez, V., Ravi Valluru, R., Prasad, P.V. & Yadav, O.P. (2016). An integrated approach to maintaining cereal productivity under climate change. Glob. Food Security, 8, pp. 9-18.

4. Feller, U. (2019). Drought stress and carbon assimilation in a warming climate: Reversible and irreversible impacts. J. Plant Physiol., 203, pp. 84-94.

5. Farooq, M., Hussain, M. & Siddique, K.H.M. (2014). Drought stress in wheat during flowering and grain-filling periods. Critical Reviews in Plant Sciences, 33, pp. 331-349.

6. Ahanger, M.A., Morad-Talab, N., Abd-Allah, E.F., Ahmad, P. & Hajiboland, R. (2016). Plant growth under drought stress: Significance of mineral nutrients. In: Water Stress and Crop Plants: A Sustainable Approach, Vol. 2, Ahma, P. (Ed.) (pp. 650-668). John Wiley & Sons, Ltd.

7. Aroca, R., Porcel, R. & Ruiz-Lozano, J.M. (2011). Regulation of root water uptake under abiotic stress conditions. J. Exp. Bot., 63, No. 1, pp. 43-57.

8. Silva, E.C., Nogueira, R.J.M.C., Silva, M.A. & Albuquerque, M. (2011). Drought stress and plant nutrition. Plant Stress, 5, No. 1. pp. 32-41.

9. Waraich, E.A., Ahmad, R., Ashraf, M.Y. & Sanullah, Eh. (2011). Role of mineral nutrition in alleviation of drought stress in plants. Aust. J. Crop Sci., 5, pp. 764-777.

10. Bityutskiy, N.P. (2011). Trace elements of higher plants. Sankt-Peterburg: Izd-vo SPb. universiteta [in Russian].

11. Khan, M., Ahmad, R., Khan, M. D., Rizwan, M., Ali, S., Khan, M. J., Azam, M., Irum, G., Ahmad, M.N. & Zhu, S. (2018). Trace Elements in Abiotic Stress Tolerance. Plant Nutrients and Abiotic Stress Tolerance, pp. 137-151.

12. Kolupaev, Yu.E. & Kokorev, O.I. (2019). Participation of polyamines in regulation of redox gomeostasis of plants. Visn. Hark. nac. agrar. univ., Ser. Biol., 46, No. 1, pp. 6-22 [in Russian].

13. Karim, M.R., Zhang, Y.Q., Zhao, R.R., Chen, X.P., Zhang, F.S. & Zou, C.Q. (2012). Alleviation of drought stress in winter wheat by late foliar application of zinc, boron, and manganese. J. Plant Nutr. Soil Sci., 175, pp. 142-151.

14. Guralchuk, Zh.Z., Trach, V.V. & Grinyuk, S.A. (2011). Efficiency of the use of microfertilizers and prospects of development of their new kinds. Bull. L'viv. Nat. Agr. Univ., 15, No. 2, pp. 98-103 [in Ukrainian].

15. Da Silva Folli-Pereira, M., Ramos, A.C., Canton, G.C., da Conceicao, J.M., de Souza, S.B., Cogo, A.J.D., Figueira, F.F., Eutropio, F.J. & Rasool, N. (2016). Foliar application of trace elements in alleviating drought stress. In Ahmad, P.(Ed.). Water Stress and Crop Plants: A Sustainable Approach., (pp. 669-681), Vol. 2, John Wiley & Sons, Ltd.

16. Thul, S.T., Sarangi, B.K. & Pandey, R.A. (2013). Nanothecnology in agroecosystem: Implication of plant productivity and its soil environment. Sci. Technol. J., 2, No. 1, pp. 1-7.

17. Pat. 38391 UA, IPC: C07C 51/41, C07F 5/00, C07F 15/00, C07C 53/126, C07C 53/10, A23L 1/00, B82B 3/00. Method of obtaining metal carboxylates "Nanotechnology for the production of metal carboxylates", Kosinov, M.V., Kaplunenko, V.G. Publ. 12.01.2009 [in Ukrainian].

18. Sokolovska-Sergiienko, O.G., Kapitanska, O.S., Priadkina, G.O. & Stasik, O.O. (2017). Antioxidant and photoprotection systems of photosynthetic apparatus in winter wheat plants treated with micronutrients, chelated by succinic acid. Fiziol. rast. genet., 49, No. 5, pp. 434-443 [in Ukrainian].

19. Davydova, O.E. & Kaplunenko, V.G. (2015). Effectiveness of new microelement complexes at winter wheat cultivation. Fiziol. rast. genet., 47, No. 3, pp. 213-223 [in Ukrainian].

20. Kapitanska, O.S., Priadkina, G.O., Stasik, O.O. & Huralchuk, Zh.Z. (2016). Relationship between parameters of photosynthetic apparatus activity and yield of winter wheat under chelated microfertilizers treatment. Fiziol. rast. genet., 48, No. 6, pp. 530-537 [in Ukrainian].

21. Kapitanska, O.S., Priadkina, G.O. & Stasik, O.O. (2018). The effect of foliar application of microelements carboxylates on photosynthetic pigments in winter wheat leaves. J. Belorus. State Univ., 2, pp. 85-94 [in Russian].

22. Morgun, V.V., Sanin, Ye.V., Shwartau, V.V. & Omelianenko, O.A. (2014). 100 centners club. Winter wheat varieties of the Institute of Plant Physiology and Genetics of the National Academy of Sciences of Ukraine and Singenta protection system. Kyiv: Logos [in Ukrainian].

23. Zadoks, J.C., Chang, T.T. & Konzak, F. (1974). A decimal code for the growth stages of cereals. Weed Research., 14, No. 6, pp. 15-21.

24. Mokronosov, A.T. & Kovalev, A.G. (Eds.) (1989). Photosynthesis and bioproductivity: methods of determination. Moscow: Agropromizdat [in Russian].

25. Gilmore, A.M., Hazlett, T.L., Debrunner, P.G. & Govindjee, G. (1996). Comparative time-resolved photosystem II chlorophyll a fluorescence analyses reveal distinctive difference between photoinhibitory reaction center damage and xanthophylls cycle-depended energy dissipation. Photochem. Photobiol., 64, No. 3, pp. 552-563.

26. Korneev, D.Yu. (2002). Information possibilities of chlorophyll fluorescence induction method. Kyiv: Altpress [in Russian].

27. Dospehov, B.A. (1973). The methods of field experiment. Moscow: Agropromizdat [in Russian].

28. Stasik, O.O. (2014). Photorespiration: Metabolism and the physiological role. In Allahverdiyev, S.I., Rubin, A.B. & Shuvalov, V.A. (Eds.). Modern photosynthetic problems (pp. 505-535), Moskva-Izhevsk: Institute of Computer Research [in Russian].

29. Voss, I., Sunil, B., Scheibe, R. & Raghavendra, A.S. (2013). Emerging concept for the role of photorespiration as an important part of abiotic stress response. Plant Biology, 15, No. 4, pp. 713-722.

30. Kiriziy, D.A., Stasik, O.O., Ryzhykova, P.L. & Trotsenko, V.A. (2017). Ontogenetic dynamics of gas exchange of top tier leaves. Fiziol. rast. genet., 49, No. 3, pp. 265-274 [in Ukrainian].

31. Pospisil, P. (2014). The role of metals in production and scavenging of reactive oxygen species in Photosystem II. Plant Cell Physiol., 55, No. 7, pp. 1224-1232.

32. Kiriziy, D.A., Stasik, O.O., Priadkina, G.O. & Shadchina, T.M. (2014). Photosynthesis: CO2 assimilation and mechanisms of its regulation. Vol. 2. Kyiv: Logos [in Russian].

33. Stasik, O.O. (2007). The response of photosynthetic apparatus of C3 plants to water deficits. Fiziologiya i biokhimiya cult. rastenii, 39, No. 1, pp. 14-27 [in Ukrainian].

34. Lawlor, D.W. & Tezara, W. (2009). Causes of decreased photosynthetic rate and metabolic capacity in water-deficient leaf cells: a critical evaluation of mechanisms and integration of process. Ann. Bot., 103, pp. 561-579.

35. Liu, H., Gan, W., Rengel, Z. & Zhao, P. (2016). Effect of zinc fertilizer rate and application method on photosynthetic characteristics and grain yield of summer maize. J. Soil Sci. and Plant Nutrition., 16, No. 2. pp. 550-562.

36. Sun, X., Hu, C., Tan, Q. & Gan, Q. (2006). Effects of molybdenum on photosynthetic characteristics in winter wheat under low temperature stress. Acta Agronomica Sinica, 32, pp. 1418-1422.

37. Karim, Md. R., Zhang, Y. Q., Zhao, R. R., Chen, X. P., Zhang, F. S. & Zou, C. Q. (2012). Alleviation of drought stress in winter wheat by late foliar application of zinc, boron, and manganese. J. Plant Nutr. Soil Sci., 175, pp. 142-151.

38. Torabian, Sh., Zahedi, M. & Khoshgoftar, A.H. (2016). Effects of foliar spray of two kinds of zinc oxide on the growth and ion concentration of sunflower cultivars under salt stress. J. Plant Nutrition, 39, No. 2, pp. 172-180.

39. Perez, C.E., Rodrigues, F.A., Moreira, W.R. & DaMatta, F.M. (2014). Leaf gas exchange and chlorophyll a fluorescence in wheat plants supplied with silicon and infected with Pyricularia oryzae. Phytopathology, 104, No. 2, pp. 143-149.

40. Yavas, I. & Unay, A. (2016). Effects of zinc and salicylic acid on wheat under drought stress. J. Anim. Plant Sci., 26, pp. 1012-1018.

41. Tabatabai, S.M.R., Oveysi, M. & Honarnejad, R. (2015). Evaluation of some characteristics of corn under water stress and zinc foliar application. Gmp Rev., 16, pp. 34-38.

42. Verbruggen, N. & Hermans, Ch. (2013). Physiological and molecular responses to magnesium nutritional imbalance in plants. Plant Soil., 368, pp. 87-99.