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Физиология растений и генетика 2017, том 49, № 2, 110-120, doi: https://doi.org/10.15407/frg2017.02.110

IRAP-аналіз генетично модифікованих рослин пшениці, отриманих за Agrobacterium- опосередкованої трансформації in vitro

Дубровна О.В., Горнчарук О.М., Великожон Л.Г.

  • Інститут фізіології рослин і генетики Національної академії наук України 03022 Київ, вул. Васильківська, 31/17

Проаналізовано рівень поліморфізму ділянок ДНК, фланкованих інвертованими LTR повторами ретротранспозонів, у генетично модифікованих рослин пшениці, отриманих методом Agrobacterium-опосередкованої трансформації в культурі in vitro, що містять ген орнітинамінотрансферази. Найбільш результативним було застосування праймера до ретротранспозону Sukkula, де в спектрі продуктів ампліфікації ДНК отримали чотири нові амплікони у дев’яти досліджених рослин. Ці дані підтвердили, що саме інсерція чужорідної ДНК здатна індукувати транспозицію ретротранспозонів Sukkula/Nikita тa Wham/Sabrina, оскільки у контрольних рослин, отриманих у культурі in vitro, їх активність не встановлена.

Ключові слова: Triticum aestivum, Agrobacterium-опосередкована трансформація, ре­тротранспозони, IRAP-ПЛР

Физиология растений и генетика
2017, том 49, № 2, 110-120

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Цитована література

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