The nitrite reductase content in leaves, and the nitrogenase activity in nodules as components of the complex of nitrogen metabolism enzymes in symbiotic system formed by soybean plants Glycine max (L.) Merr. of the Samorodok variety and nodule bacteria Bradyrhizobium japonicum РС08 using nanocarboxylates of germanium and molybdenum under salinity (0.25 g NaCl/kg of sand) conditions were revealed. It was found that the content of nitrite reductase in soybean plants inoculated with rhizobia cultivated with trace elements was higher than in control plants bacterized with a suspension of nodule bacteria without nanocarboxylates. Salinity caused a decrease in the amount of nitrate reductase in leaves, and nitrogenase activity in plants of all studied variants, but changes in these indices varied depending on the composition of the inoculation suspension. The smallest difference in both enzymes between plants under salinity and the corresponding control was found with the use of Mo, and the largest — with Ge. The obtained data expand the understanding the Mo and Ge influence on the nitrogen assimilation enzymes activity, and support the use of Mo to optimize nitrogen metabolism in soybean plants in symbiosis with nodule bacteria under salinity conditions.
Keywords: Glycine max (L.) Merr., Bradyrhizobium japonicum, inoculation, nitrіte reductase, nitrogen-fixing activity, salinity
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