Fiziol. rast. genet. 2024, vol. 56, no. 3, 213-229, doi: https://doi.org/10.15407/frg2024.03.213

Cytogenetic analysis of induced mutations in the exclusion zone of Chernobyl NPP 35 years after the accident

Yakymchuk R.A., Morgun V.V., Skriplov V.O.

  • Institute of Plant Physiology and Genetics, National Academy of Sciences of Ukraine

The mutagenic activity of radionuclide soil contamination in the exclusion zone of the Chernobyl nuclear power plant 35 years after the accident was studied. Using the ana-telophase method, a cytogenetic analysis of root meristem cells of Triticum aestivum L. seedlings of Smuglyanka and Bohdanа varieties, which were treated by prolonged exposure to radionuclide soil contamination within the 10-km zone of the Chernobyl NPP (the villages of Kopachi, Chistohalivka, and Yaniv), was carried out. The specific activity of 137Cs and 90Sr was 4.5—28.2 kBq/kg. The soil of Glevakha village, Fastiv district, Kyiv region, whose specific radioactivity is 0.29 kBq/kg, was taken as control. The frequency of aberrant cells induced by radionuclide soil contamination exceeds the control level by 3.5—7.5 times. A direct relationship between the frequency of aberrant cells and the specific radioactivity of the soil was not found. The spectrum of chromosomal disorders included paired fragments and bridges, typical for the conditions of exposure to ionizing radiation, the ratio of which varies between 1.0 and 7.2. The mutagenic effect of radionuclide contamination of the soil causes an expansion of the chromosomal aberrations spectrum and abnormalities of mitosis, which includes chromosomal acentric rings, micronuclei, lagging chromosomes. Induction of lagging chromosomes as a result of prolonged exposure to radionuclide contamination of the soil indicates the aneugenic effect of the mutagenic factor. The mutagenic effect of radionuclides in the soil of the Chernobyl NPP exclusion zone is accompanied by the appearance of cells with multiple aberrations, the proportion of which shows an inverse dependence on the density of radionuclide contamination, which can be taken into account when conducting radioecological monitoring of the natural environment. The results of long-term research of the genetic consequences of the Chernobyl disaster and accidents at nuclear objects in other countries indicate a long-term radiation threat to the stability of the genome of living organisms and serve as a basis for including in the state environmental program systematic genetic monitoring of the territories affected by radionuclide pollution and near radiation-dangerous objects.

Keywords: Triticum aestivum L., radionuclide contamination, specific radioactivity, chromosomal aberrations

Fiziol. rast. genet.
2024, vol. 56, no. 3, 213-229

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