Fiziol. rast. genet. 2022, vol. 54, no. 2, 123-133, doi:

Physiological and biochemical changes in soybeen plants under influence of phytopathogenic microorganisms and presowing treat­ment of seeds with molibdenum nanochelates

Huliaieva H.B., Tokovenko I.P., Hnatiuk T.T., Bogdan M.M., Paty­ka V.P.

  • D.K. Zabolotny Institute of Microbiology and Virology, National Academy of Sciences of Ukraine 154 Academika Zabolotnogo St., Kyiv, 03143, Ukraine

Under the conditions of pot experiment, the effect of soybean plants inoculation by various phytopathogens (WSMV, Acholeplasma laidlawii and Xantomonas axonopodis pv. glycines) and pre-sowing treatment with nanochelates of Mo was studied. Inhibition of leaves photosynthetic activity was established in all experimental variants. At the same time, the dark respiration rate increased in the variants with WSMV inoculation and bacterial pathogen, and presowing treatment with nanochelates of Mo and inoculation with WSMV. The leaf transpiration increased most significantly under inoculation of X. axonopodis pv. glycines and most significantly decreased under inoculation WSMV, as well as in variants presowing Mo treatment + WSMV, and presowing Mo treatment + X. axonopodis pv. glycines. In leaves of plants infected by phytoplasma and bacterial pathogen, content of phytohormones IAA and ABA decreased, and infected with WSMV — increased significantly. However, under presowing treatment of soybean by Mo nanochelates reduced content of both phytohormones in leaves was observed, which can be explained by the prolonged effect of elevated air temperature during the growing season, that during day reached 30 °C or more. Presowing treatment with Mo nanochelates under inoculation by phytoplasma and WSMV caused a decrease in the IAA and ABA content in leaves, while bacterial inoculation caused the decrease of IAA content with increase of ABA content in the leaves. Increase of phenolic compounds content in leaf tissues was observed in all experimental variants. Thus, under the conditions of combined stress, caused by elevated temperatures and phytopathogenic infection, treatment with Mo nanochelates performed a regulatory function in relation to plant metabolism, helping to increase resistance to the destructive effects of phytopathogenic infection.

Keywords: Acholeplasma laidlawii, phytoplasma, WSMV, Xantomonas axonopodis pv. glycines, photosynthesis, phytohormones, phenolic compounds

Fiziol. rast. genet.
2022, vol. 54, no. 2, 123-133

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