Fiziol. rast. genet. 2020, vol. 52, no. 6, 463-468, doi:

Lectin activity of soybean seeds

Kyrychenko O.V.

  • Institute of Plant Physiology and Genetics, National Academy of Sciences of Ukraine
    31/17 Vasylkivska St., Kyiv, 03022, Ukraine

The lectin activity of soybean seeds of both the sowing seeds and the yield seeds received by the pretreatment of sowing seeds with the purified soybean lectin as well as the lectin-rhizobium compositions on the bases of the soybean lectin and nodule bacteria Bradyrhizobium japonicum 634b was studied. It has been demonstrated that the application of compositions with high lectin concentrations (50 and 500 mg/ml) increased lectin activity of yield seeds, whereas a composition with a low lectin content (5 mg/ml) doesn’t increase lectin activity of yield seeds as compared with the sowing material (parental seeds). Application of a purified soybean lectin in concentration 500 mg/ml for the pre-sowing treatment of soybean seeds essentially increased lectin activity of yield seeds. We suggest using of soybean seed lectin at the concentration of 5 mg/ml to modify the rhizobial inoculant for soybean in order to minimize the accumulation of proteins with lectin activity in soybean yield seeds.

Keywords: soybean, lectin, compositions, real lectin activity, specific lectin activity

Fiziol. rast. genet.
2020, vol. 52, no. 6, 463-468

Full text and supplemented materials

Free full text: PDF  


1. Rudiger, H. & Gabius, H.-J. (2001). Plant lectins: occurrence, biochemistry, functions and applications. Glycoconjugate J., 18, No. 8, pp. 589-613.

2. Van Damme, E.J.M., Lannoo, N. & Peumans, W.J. (2008). Plant Lectins. Adv. Botanical Res., 48. pp. 107-209.

3. Nikitina, V.E., Loshchinina, E.A. & Vetchinkova, E.P. (2017). Lectins from mycelia of basidiomycetes. Int. J. Mol. Sci., 18, No. 7, pp. 1334-1350.

4. Singh, K., Kaur, M., Rup, P.J. & Singh, J. (2006). Exploration for anti-insect properties of lectin from seeds of soybean (Glicine max) using Bactrocera cucurbitae as a model. Phytoparasitica, 34, No. 5, pp. 463-473.

5. Jiang, S., Ma, Z. & Ramachandran, S. (2010). Evolutionary history and stress regulation of the lectin superfamily in higher plants. BMC Evol Biol., 10, No 1, pp. 79-103.

6. Alenkina, S.A., Bogatyrev, V.A., Matora, L.Y., Sokolova, M.K., Chernyshova, M.P., Trurneva, K.A. & Nikitina, V.E. (2014). Signal effects of the lectin from the associative nitrogen-fixing bacterium Azospirillum brasilense Sp7 in bacterial-plant root interactions. Plant Soil. No. 381, pp. 337-349.

7. Kyrychenko, O.V. (2014). Phytolectins and diazotrophs are the polyfunctional components of the complex biological compositions. Biotechnol Acta. 7, No 1, pp. 40-53 [in Ukrainian].

8. Pavlovskaya, N.E. & Gagarina, I.N. (2017). The physiological properties of plant lectins as a prerequisite for their application in biotechnology. Khimiya rastitelnogo syriya. No. 1, pp. 21-35 [in Russian].

9. Hendrickson O.D. & Zherdev, A.V. (2018). Analytical application of lectins. Critical Rev in Analytical Chemistry, 48, No. 4, pp. 279-292.

10. Sharon, N. (2007). Lectins: carbohydrate-specific reagents and biological recognition molecules. J. Biol. Chem., 282, No. 5, pp. 2753-2764.

11. Gabius, H.J. (2018). The sugar code: why glycans are so important. Bio Systems. 2018. 164, pp. 102-111.

12. Antonyuk, V.O. (2005). Lectins and their raw material sources. PP «Kvart»: Lviv [in Ukrainian].

13. Aoyama, H., Cavagis, A.D.M., Taga, E.M. & Ferreira, C.V. (2001). Endogenous lectin as a possible regulator of the hydrolysis of physiological substrates by soybean seed acid phosphatase. Phytochemistry, 58, No. 2, pp. 221-225.

14. Kyrychenko, O.V. (2008). Practice of soybean and wheat lectins use for the plant growing. Problems of Biogeochem. Geochemical Ecology, 1, pp. 99-105.

15. Kyrychenko, O.V. (2011). Changes in endogenous lectin activity of soybean plants under seeds treatment with exogenous lectin. Fyzyolohyia i byokhymyia kult. rasteniy, 43, No. 6, pp. 520-526 [in Ukrainian].

16. Hartley, M.R. & Lord, J.M. (2004). Cytotoxic ribosome-inactivating lectins from plants. Biochem. Biophys. Acta., 1707, No. 1-2, pp. 1-14.

17. Li, Z., Li, D. & Qiao, S. (2003). Effect of soybean agglutinin on nitrogen metabolism and on characteristics of intestinal tissues and pancreas in rats. Arch. Tierernahr., 57, No. 5, pp. 369-380.

18. Hisayasu, S., Orimo, H., Migita, S., Ikeda, Y., Satoh, K., Shinjo, S., Hirai, Y. & Toshino, Y. (1992). Soybean protein isolate and soybean lectin inhibit iron absorption in rats. J. Nutr., 122, No. 5, pp. 1190-1196.

19. Whitaker, J.R. & Einar, G. (1980). An absolute method for protein determination based on difference in absorbance at 235 and 280 nm. Anal. Biochem., 109, No. 1, pp. 156-159.

20. Lodeiro, A.R., Lopez-Garcia, S.L., Vazquer, T.E.E. & Favelukes, G. (2000). Stimulation of adhesiveness, infectivity and competitiveness for nodulation of Brady­rhizobium japonicum by its pretreatment with soybean seed lectin. FEMS Microbiol. Let., 188, No. 1, pp. 177-184.

21. Kandelinskaya, O.L., Grischenko, E.R., Ripinskaya, K.Ju., Aleschenkova, Z.M., Kartizhova, L.E., Kuptsov, V.N. & Kuptsov, N.S. (2015). Role of lectins in regulation of legume-Rhizobium symbiosis efficiency in lupin. Botanika (issledovaniya). Sb. Nauch. Tr.: In-t Experimentalnoy Botaniki of the National Academy of Scienses of Belarusi, 44, pp. 283-290 [in Russian].