Fiziol. rast. genet. 2020, vol. 52, no. 5, 449-460, doi:

Peculiarities of the genus Clematis L. plants water regime

Kovalyshyn I.B.

  • Institute of Plant Physiology and Genetics, National Academy of Sciences of Ukraine 31/17 Vasylkivska St., Kyiv, 03022, Ukraine

Clematis is a medicinal plant and a promising culture for introduction into urbophytocenoses. In order to ensure optimal growing conditions, it is necessary to take into account the ecological needs of plants, a key place among which is water supply. The experiments were carried out with cultivars and species of the genus Clematis: Clematis alpina ‘Pamela Jackman’, C. macropetala ‘Maidwell Hall’, C. integrifolia ‘Aljonushka’, C. ispahanica ‘Zvezdograd’, C. fargesii ‘Paul Farges’, C. taxensis ‘Princess Diana’, C. tibetana, C. viticella and C. heracleifolia. Ecological peculiarities of studied plants cause differences in the anatomical tissues structure and dynamic of physiological processes. The lowest content of dry matter in the leaves was found in C. alpina ‘Pamela Jackman’ (16,3 %) and C. macropetala ‘Maidwell Hall’ (18,3), indicating their potentially lowest drought resistance. The highest value of this index was identified in C. heracleifolia (27.5 %) and C. integrifolia ‘Aljonushka’ (23.4 %), that characterizes them as the most drought resistant among studied plants. A function describing the process of cutted leaves wilting at the temperature +60 °C was defined: f(x) = y0 + ae–bx. A significant correlation was found between the parameter y0 and the dry mass (0.99), water content (0.86) in the leaves and their weight before drying (0.91); the parameter a and the mass index of leaves before wilting (0.99), the water content (1.00) and dry matter (0.83), as well as the density (0.86) and the area of stomatas (0.81) on the adaxial surface of leaves. Parameter b correlated with the values of dry mass (—0.75), its fraction in the total leaf mass (—0.84), the water fraction (0.83) and the width of spongy mesophyll cells (0.76). The peculiarities of anatomical structure and dynamic of leaves water loss process among investigated plants indicate the difference in their potential drought resistance. Obtained results allow to choose the optimal conditions for growing of investigated plants of the genus Clematis and planning additional care measures.

Keywords: Clematis L, drought resistance, drying dynamics, anatomical structure of leaves, dry matter

Fiziol. rast. genet.
2020, vol. 52, no. 5, 449-460

Full text and supplemented materials

Free full text: PDF  


1. Tamura, M. (1993). Ranunculaceae. In The Families and Genera of Vascular Plants. Flowering Plants Dicotyledons. Berlin: Springer 2, pp. 563-583.

2. Troomey, M. & Leeds, E. (2001). An Illustrated Encyclopedia of Clematis. London: Timber Press.

3. Walter, H. (2012). Vegetation of the Earth and Ecological Systems of the Geo-biosphere. New York: Springer Science & Business Media.

4. Zhang, H.-X., Zhang, M.-L. & Sanderson, S.C. (2013). Retreating or Standing: Responses of Forest Species and Steppe Species to Climate Change in Arid Eastern Central Asia. PLoS One, 8 (4), p. e61954.

5. Komatsu, S. (2019). Plant Proteomic Research 2.0: Trends and Perspectives. Int. J. Mol. Sci., 20 (10), p. 2495.

6. Moon, A.R., Han, J.E., Lee, B.Y., Park, J.M. & Jang, C.G. (2013). An Unrecorded Species of Genus Clematis (Ranunculaceae) from Korea. Journal of Asia-Pacific Biodiversity, 6 (4), pp. 415-418.

7. Grey-Wilson, Ch. (2002). Clematis: The Genus: A Guide for Gardeners, Horticulturists and Botanists. London: Batsford.

8. Chawla, R., Kumar, S. & Sharma, A. (2012). Pharmacognostic Standardization of Clematis erecta Linn. International Journal of Pharmacy and Pharmaceutical Sciences, 4 (1), pp. 482-487.

9. Chawla, R., Kumar, S. & Sharma, A. (2012). The genus Clematis (Ranunculaceae): Chemical and pharmacological perspectives. Journal of Ethnopharmacology, 143, pp. 116-150.

10. Karimi, E., Ghorbani Nohooji, M., Habibi, M., Ebrahimi, M., Mehrafarin, A. & Khalighi-Sigaroodi, F. (2018). Antioxidant potential assessment of phenolic and flavonoid rich fractions of Clematis orientalis and Clematis ispahanica (Ranunculaceae). Natural product research, 32 (16), pp. 1991-1995.

11. Naika, H.R., Bhavana, S., Lingaraju, K., da Silva, J.A.T. & Krishna, V. (2019). Antiproliferative, Antioxidant and Hepatoprotective Activities of Clematis gouriana Roxb. Extracts. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences, 89 (3), pp. 921-929.

12. Mostafa, M., Ahmed, S. & Afolayan, A.J. (2018). Antioxidant activity of Clematis brachiata Thunb. leaf. Bangladesh Journal of Scientific and Industrial Research, 53 (3), pp. 185-190.

13. Morgun, V.V., Kiriziy, D.A. & Shadchina, T.M. (2010). Ecophysiological and genetical aspects of crops adaptation to global climate changes. Fiziologiya i biokhimiya kult. rasteniy, 42 (1), pp. 3-22 [in Russian].

14. Morgun, V.V., Stasyk, O.O., Kiriziy, D.A. & Pryadkina, G.O. (2016). Relarions between reactions of photosynthetic traits and grain productivity on soil drought in winter wheat varieties contrasting in their tolerance. Fiziol. rast. genet., 48 (5), pp. 371-381. [in Ukrainian].

15. Kondratyuk, Yu.Yu., Rybachenko, O.R., Mamenko, P.M. & Kots, S.Ya. (2015). The influence of drought on the protein content of soebean roots under inoculation by Bradyrhizobium japonicum strains with different effectivness. Fiziol. rast. genet., 47 (4), pp. 361-366 [in Ukrainian].

16. Chavarria, G. & dos Santos, H.P. (2012). Plant water relations: absorption, transport and control mechanisms. Advances in Selected Plant Physiology Aspects. London: IntechOpen, pp. 105-132.

17. Reynolds, M.P., Pask, A.J.D. & Mullan, D.M. (Eds.) (2012). Physiological Breeding I: Interdisciplinary Approaches to Improve Crop Adaptation. Mexico, D.F.: CIMMYT.

18. Koelling, C. (2016). Plant Anatomy, Morphology and Physiology. New York: Syrawood Publishing House.

19. Javed, A., Ahmad, M., Zafar, M., Sultana, S., Khan, M.A. & Arshad, M. (2012). Palyno-Anatomical Studies of Clematis L. (Ranunculaceae) from Poonch Valley Ajk, Pakistan. The Journal of Animal & Plant Sciences, 22 (4), pp. 1173-1176.

20. Li, M., He, J., Zhao, Z., Lyu, R., Yao, M., Cheng, J. & Xie, L. (2020). Predictive modelling of the distribution of Clematis sect. Fruticella s. str. under climate change reveals a range expansion during the Last Glacial Maximum. Peer J. 8, p. e8729.

21. Yang, Y., Guo, X., Wang, K.L., Liu, Q. H. & Liu, Q.C. (2019). Anther and ovule development in Clematis terniflora var. mandshurica (Ranunculaceae). Flora, 253, pp. 67-75.

22. Systat Software Inc. (2010). SigmaPlot 12: User's Guide. Systat Software Incorporated.