Section «Plant Growth and Development, Physiologically Active Substances» of the Ukrainian Society of Plant Physiologists unites scientists of the Phytohormonology Department and the Department of Membranology and Phytochemistry of the M.G. Kholodny Institute of Botany of NAS of Ukraine. The section includes four doctors and fifteen candidates of biological sciences. Studies in the field of phytophysiology have established patterns of the phytohormonal system functioning in fern sporophytes during ontogenesis, and determined the optimal concentrations of exogenous phytohormones for initiating spore germination and gametophyte development in vitro culture. Triticum aestivum and T. spelta grain priming with exogenous abscisic acid (ABA) has been proved to cause resistance to hyperthermia and moderate soil drought, as well as to accelerate recovery from stresses. The cereals growth dynamics under hyperthermia and soil drought conditions, and during the recovery period was shown to be regulated by the balance and localization of endogenous ABA and indole-3-acetic acid. The biotechnological basis for the production of biological preparations with a high cytokinin activity from the mycelial biomass of medicinal mushrooms has been developed for use in medicine. The biotechnological winter wheat priming with a new preparation based on bacterial mediators — acylhomoserinlactone was tested in the field. The invention patent was granted. Studies of phytochemists have established that stromal carbonic anhydrases (CA) of chloroplasts have different sensitivity to heavy metals. The data obtained confirm the possibility of using chloroplasts CA for the early monitoring of environmental pollution. The existence of regulatory sites in the ATP-synthase complex of chloroplasts that are sensitive to the level of HCO3-/CO2 in the medium was proved. Sucrose was demonstrated to be involved in the adaptation of Galanthus nivalis photosynthetic apparatus to low temperatures. A measuring unit for CO2 ecosystem flows based on the CO2 gas analyzer S-151 was installed to assess the effects of environmental pollution with oil products and heavy metals.
Keywords: phytohormonology, phytochemistry, membranology, biotechnology, cereals, photosynthesis, heavy metals
Full text and supplemented materials
Free full text: PDFReferences
1. Al-Maali, G.A., Vedenicheva, N.P., Bisko, N.A. & Kosakivska, I.V. (2019). Effect of microelements on cytokinins content in mycelial biomass of medicinal mushroom Trametes versicolor (Polyporaceae, Basidiomycota). Ukranian Botanical Journal, 76 (1), pp. 71-78 [in Ukrainian]. https://doi.org/10.15407/ukrbotj76.01.071
2. Belyavskaya, N.A., Fediuk, O.M. & Zolotareva, E.K. (2018). Plants and Heavy Metals: Reception and Signaling. Visn. Kharkiv. nat. agrarian. un-tu. Ser. Biol., 3(45), pp. 10-30 [in Ukrainian].
3. Bilyavska, N.O. & Fediuk, O.M. (2018). Leaf surface microstructure of Galanthus nivalis L. at early-spring stages of ontogenesisi. Kharkiv. nat. agrarian. un-tu. Ser. Biol., 2(44), pp. 50-58 [in Ukrainian]. https://doi.org/10.35550/vbio2018.02.050
4. Vedenicheva, N.P., Al-Maali, G.A., Bisko, N.A., Shcherbatiuk, M.M. & Kosakivska, I.V. (2018). Peculiarities of the growth and the content of endogenous cytokinins in the micelial biomass of basidial mushrooms Hericium coralloides and Fomitopsis officinalis growing in vitro. Dopov. Nac. akad. nauk Ukr., No. 9, pp. 97-104 [in Ukrainian]. https://doi.org/10.15407/dopovidi2018.09.097
5. Kosakivska, I.V., Vasyuk, V.A. & Voytenko, L.V. (2018). Endogenous phytogormones in leptosporagiate fern Dryopteris filix-mas (L.) Schott. Dopov. Nac. akad. nauk Ukr., No. 12, pp. 79-86 [in Ukrainian]. doi.org/10.15407/dopovidi2018.12.079 https://doi.org/10.15407/dopovidi2018.12.079
6. Kosakivska, I.V., Babenko, L.M., Vashaka, O.V., Vasyuk, V.A., Voytenko, L.V., Romanenko, K.O. & Shcherbatiuk, M.M. (2019). Phytohormonal system and structural and functional features of fern (Polypodiaphyta). I.V. Kosakivska. (Ed). Kyiv: Nash Format, 250 p. [in Ukrainian].
7. Kosakivska, I.V., Romanenko, K.O., Voytenko, L.V., Vasyuk, V.A., Shcherbatiuk, M.M. & Babenko, L.M. (2019). Hormonal complex of gametophytes of Dryopteris filix-mas (Dryopteridaceae) in vitro culture. Ukr. Botan. J., 76(3), pp. 260-269 [in Ukrainian]. https://doi.org/10.15407/ukrbotj76.03.260
8. Kosakivska, I.V., Voytenko, L.V., Vasyuk, V.A., Vedenichova, N.P., Babenko, L.M. & Shcherbatyuk, M.M. (2019). Phytohormonal regulation of seed germination. Fiziol. rast. genet., 51(3), pp. 187-206 [in Ukrainian]. https://doi.org/10.15407/frg2019.03.187
9. Kosakivska, I.V., Vasyuk, V.A. & Voytenko, L.V. (2019). Effect of exogenous abscisic acid on morphological characteristics of winter wheat and spelt under hyperthermia. Fiziol. rast. genet., 51(4), pp. 324-337 [in Ukrainian]. https://doi.org/10.15407/frg2019.04.324
10. Kosakivska, I.V., Shcherbatiuk, M.M., Vasyuk, V.A. & Voytenko, L.V. (2019). Hormonal system of plants under the action of heavy metals. Kharkiv. nat. agrarian. un-tu. Ser. Biol., 3(48), pp. 6-22 [in Ukrainian]. https://doi.org/10.35550/ vbio2019.03.006
11. Mokrosnop, V.M. (2019). The effect of light intencity on the growth of mixotrophic cultures of Euglena gracilis and photosynthetic pigments accumulation in cells. Fiziol. rast. genet., 51(1), pp. 76-83 [in Ukrainian]. https://doi.org/10.15407/frg2019.01.076
12. Pat. 120310, Method of processing seeds and vegetative mass of winter wheat plants, Joined 11. 11. 2019.
13. Polishchuk, A.V., Semenikhin, A.V., Topchyi, N.M. & Zolotareva, E.K. (2018). Inhibition of multiple forms of carbonic anhydrases of spinach chloroplasts by Cu ions. Dopov. Nac. akad. nauk Ukr., No. 4, pp. 94-101 [in Ukrainian]. https://doi.org/10.15407/dopovidi2018.04.094
14. Syvash, O.O., Mykhaylenko, N.F. & Zolotareva, E.K. (2018). Variation of chlorophyll a to b ratio at adaptation of plants to external factors. Visn. Hark. nac. agrarian un-tu, Ser. Biol., 3(45), pp. 49-73 [in Ukrainian]. https://doi.org/10.35550/vbio2018.03.049
15. Stepanov, S.S. & Polishchuk, O.V. (2019). Influence of 2-(2-oxypyrolidin-1-il)acetamide on growth and functional state of photosynthetic apparatus of Chlamydomonas reinhardtii. Fiziol. rast. genet., 51(3), pp. 267-275 [in Ukrainian]. https://doi.org/10.15407/frg2019.03.267
16. Topchiy, N.M., Polishchuk, O.V., Zolotareva, E.K. & Sytnyk, S.K. (2019). The influence of Cd2+ ions on the activity of stromal carbonic anhydrases of spinach chloroplasts. Fiziol. rast. genet., 51(2), pp. 172-182 [in Ukrainian]. https://doi.org/10.15407/frg2019.02.172
17. Babenko, L.M., Hospodarenko, H.M., Rozhkov, R.V., Pariy, Ya.F., Pariy, M.F., Babenko, A.V. & Kosakivska, I.V. (2018). Triticum spelta L.: origin, biological characteristics and perspectives of use in breeding and agriculture. Regulatory Mechanisms in Biosystems, 8(2), pp. 250-257. https://doi.org/10.15421/021837
18. Babenko, L., Vasheka, O., Shcherbatiuk, M., Romanenko, P., Voytenko, L. & Kosakivska, I. (2019). Biometric characteristics and surface microstructure of vegetative and reproductive organs of heterosporous water fern Salvinia natans. Flora, 252, pp. 44-50. https://doi.org/10.1016/j.flora.2019.02.006
19. Babenko, L.M., Smirnov, O.E., Romanenko, K.O., Trunova, O.K. & Kosakivska, I.V. (2019). Phenolic compounds in plants: biogenesis and functions. Ukr. вiochem. J., 91(3), pp. 5-18. https://doi.org/10.15407/ubj91.03.005
20. Babenko, L.M., Vodka, M.V., Akimov, Yu. N., Smirnov, A.E., Babenko, A.V. & Kosakovskaya, I.V. (2019). Specific features of the ultrastructure and biochemical composition of Triticum spelta L. leaf mesophile cells in the initial period of stress temperature action. Cell and Tissue Biology, 13(1), pp. 70-78. https://doi.org/10.1134/S1990519X19010024
21. Bilyavska, N.O., Fediuk, O.M. & Zolotareva, E.K. (2019). Chloroplasts of cold-tolerant plants. Plant Science Today, 6(4), pp. 407-411. https://doi.org/10.14719/pst.2019.6.4.584
22. Fediuk, O.M., Bilyavska, N.O. & Zolotareva, E.K. (2018). Effects of soil early-spring temperature on the morphometric parameters of mitochondria in Galanthus nivalis L. leaves. Plant Science Today, 5(4), pp. 149-154. https://doi.org/10.14719/pst.2018.5.4.405
23. Kosakivska, I.V., Voytenko, L.V., Vasyuk, V.A. & Shcherbatiuk, M.M. (2019). Effect of zinc on growth and phytohormones accumulation in Triticum aestivum L. priming with abscisic acid. Dopov. Nac. akad. nauk Ukr., No. 11, pp. 93-99. https://doi.org/10.15407/dopovidi2019.11.093
24. Kosakivska, I.V., Vasyuk, V.A., Voytenko, L.V., Shcherbatiuk, M.M., Romanenko, K.O. & Babenko, L.M. (2020). Endogenous phytohormones of fern Polystichum aculeatum (L.) Roth. gametophytes at different stages of morphogenesis in vitro culture. Cytology and Genetics, 54(1), pp. 23-30. https://doi.org/10.3103/S0095452720010089
25. Mokrosnop, V.M. (2018). Production of storage polysaccharide paramylon in microalga Euglena gracilis Klebs (Euglena, Euglenophyceae). Int. J. Algae, 20(2), pp. 153-166. https://doi.org/10.1615/InterJAlgae.v20.i2.40
26. Moshynets, O.V., Babenko, L.M., Rogalsky, S.P., Iungin, O.S., Foster, J., Kosakivska, I.V., Potters, G. & Spiers, A.J. (2019). Priming winter wheat seeds with the bacterial quorum sensing signal N-hexanoyl-L-homoserinelactone (C6-HSL) shows potential to improve plant growth and seed yield. PLoS One. 14 (2), pp. 1-17. https://doi.org/10.1371/journal.pone.0209460
27. Onoiko, O.B., Khomochkin, A.P. & Zolotareva, O.K. (2019). Influence of sulfite on ATPase activity of coupling factor CF1 isolated from spinach chloroplasts. Ukr. Biochem. J., 9(1), pp. 47-52. https://doi.org/10.15407/ubj91.01.047
28. Romanenko, K.O., Kosakivska, I.V., Babenko, L.M., Vasheka, O.V., Romanenko, P.O., Negretsky, V.A. & Minarchenko, V.M. (2019). Effects of exogenous cytokinins on spore germination and gametophyte morphogenesis of Dryopteris filix-mas (L.) Schott in vitro culture. Cytol. Genet, 53(3), pp. 192-201. https://doi.org/10.3103/S0095452719030034
29. Vasyuk, V.A., Voytenko, L.V., Shcherbatiuk, M.M. & Kosakivska, I.V. (2019). Effect of exogenous abscisic acid on seed germination and growth of winter wheat seedlings under zinc stress. J. Stress Physiol & Biochem., 15(2), pp. 68-78.
30. Vedenicheva, N.P., Al-Maali, G.A., Bisko, N.A., Shcherbatiuk, M.M., Lomberg, M.L., Mytropolska, N.Y., Mykchaylova, O.B. & Kosakivska, I.V. (2018). Comparative Analysis of Cytokinins in Mycelial Biomass of Medicinal Mushrooms. Int. J. Medic. Mushrooms, 20(9), pp. 837-847. https://doi.org/10.1615/IntJMedMushrooms.2018027797
31. Vedenicheva, N.P., Al Maali, G.A., Mykhaylova, O.B., Lomberg, M.L., Bisko, N.A., Shcherbatiuk, M.M. & Kosakivska, I.V. (2018). Endogenous Cytokinins Dynamics in Mycelial Biomass of Basidiomycetes at Different Stages of Cultivation. Int. J. Biochem. Physiol., 3(2), pp. 1-5. https://doi.org/10.23880/IJBP-16000122
32. Vedenicheva, N., Al-Maali, G., Bisko, N., Kosakivska, I., Garmanchuk, L. & Ostapchenko, L. (2019). Effect of bioactive extracts with high cytokinins content from mycelial biomass of Hericium coralloides and Fomitopsis officinalis on tumor cells in vitro. Bulletin of Taras Shevchenko National University of Kyiv. Ser. Biology, 79(1), pp. 20-25. https://doi.org/10.17721/1728_2748.2019.79.31-37
33. Zolotareva, E.K., Mokrosnop, V.M. & Stepanov, S.S. Polyphenol compounds of macroscopic and microscopic algae. Int. J. Algae, 21(1), pp. 5-24. https://doi.org/10.1615/InterJAlgae.v21.i1.10