Fiziol. rast. genet. 2018, vol. 50, no. 1, 66-76, doi: https://doi.org/10.15407/frg2018.01.066

POLYMORPHISM OF SOFT WHEAT VARIETIES BY LOCI OF LOW MOLECULAR WEIGHT GLUTENINS

Radchenko O.M.

  • Institute of Plant Physiology and Genetics, National Academy of Sciences of Ukraine 31/17 Vasylkivska St., Kyiv, 03022, Ukraine

Using allele-specific primers, the allelic state of the Glu-A3, Glu-B3, Glu-D3 loci of 35 soft wheat varieties was determined by PCR-analysis. Two alleles were detected in the Glu-A3 locus: Glu-A3d, Glu-A3c; Glu-A3c allele prevailed. Four alleles were identified in the Glu-B3 locus: Glu-B3b, Glu-B3j, Glu-B3g, Glu-B3d, the allele Glu-B3b predominated. The index of sedimentation in the investigated samples was determined. In samples that had the allele Glu-B3g, the average sedimentation index was 85, in samples having an allele of Glu-B3b, 80. The alleles of the Glu-B3 locus can be divided according to their effect on the sedimentation index: g > b > j.

Keywords: soft winter wheat, PCR-analysis, low molecular weight glutenins loci

Fiziol. rast. genet.
2018, vol. 50, no. 1, 66-76

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