An effective method of Vaccinium vitis-idaea L. genetic transformation has been elaborated. In this method the type of the explant, the vir-gene inductors, the explants’ sensitivity to selective and bacteriostatic antibiotics, and the strategy of the transformants selection have been defined. Transgenic plants of Vaccinium vitis-idaea L. (Red Pearl cultivar) have been received for the first time.
Keywords: Vaccinium vitis-idaea L., Agrobacterium tumefaciens, genetic transformation, expression gene, PCR-analysis
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1. Dreyper, Dzh., Skott, R. & Armitidzh, F. (1991). Plant genetic engineering: a laboratory manual: Laboratornoe rukovodstvo. M.: Mir 34 [in Russian].
2. Sivolap, Yu.M. (1998). The use of PCR analysis in genetic and breeding studies. Kyiv: Agrarna nauka [in Russian].
3. Chan, M.T., Chang, H.H., Ho, S.L.,Tong, W.F. & Yu, S.M. (1993). Agrobacterium-mediated production of transgenic rice plants expressing a chimeric alpha-amylase promoter/beta-glucuronidase gene. Plant Mol. Biol., 22, pp. 491-506. https://doi.org/10.1007/BF00015978
4. http://www.biotechnolog.ru/ge/ge12_4.htm, 06.03.2017.
5. Jefferson, R.A. (1987). GUS-fusion: b-glucuronidase as a sensitive and versalite gene fusion marker in higher plants. EMBO J., 6, pp. 3901-3907. https://doi.org/10.1002/j.1460-2075.1987.tb02730.x
6. Kanik, T., Tzfira, T., Kapulnik, Y.,Gafni, Y., Dinqwall, C. & Citovsky, V. (2001). Genetic transformation of HeLa cells by Agrobacterium. Proc. Natl. Acad. Sci. USA., 98, pp. 1871-1876. https://doi.org/10.1073/pnas.98.4.1871
7. Lloyd, G. & McCown, B. (1980). Commercially feasible micropropagation of mountain laurel, Kalmia latifolia, by use of shoot tip culture. Comb. Proc. Intl. Plant Prop. Soc., 30, pp. 421-427.
8. Potrykus, I. (1991). Gene transfer to plant: Assessment of published approaches and result. Annu. Rev. Plant Physiol. Plant Mol. Biol., 42, pp. 205-225. https://doi.org/10.1146/annurev.pp.42.060191.001225