Fiziol. rast. genet. 2018, vol. 50, no. 2, 115-123, doi: https://doi.org/10.15407/frg2018.02.115

PHYSIOLOGICAL PECULIARITIES OF SUGAR BEET REGENERANTS LONG-TERM CULTIVATED IN VITRO

Klyachenko O.L.

  • National University of Life and Environmental Sciences of Ukraine 15 Heroiv Oborony St., Kyiv, 03041, Ukraine

Specific and non-specific quantitative and qualitative changes of concentration of photosynthetic pigments (chlorophylls a, b, and carotenoids), activity of anion peroxidases and content of phenolic compounds were detected in sugar beet regenerants, which were cultivated for a long time under in vitro conditions. This features play important role in regulatory and defense mechanisms and shown significant genotypic differences between regenerants. A cluster analysis for the similarity of genotypes was carried out by a quantitative biochemical state of the metabolic profile. These peculiarities are revealed in respect to the formation of ecological plasticity of plants, which have great importance for increasing the efficiency of the sugar beet breeding process.

Keywords: sugar beet, triterpene glycosides, flavonoids, peroxidases, metabolic profile

Fiziol. rast. genet.
2018, vol. 50, no. 2, 115-123

Full text and supplemented materials

Free full text: PDF  

References

1. Balkov, I.Ya. (2011). A new stage in selection and seed-growing - highly profitable hybrids Saharnaja svekla, No. 7, pp. 26-28 [in Russian].

2. Bojarkin, A.M. (1951). A quick method for determining peroxidase activity. Biohimija, 16(4), pp. 325-355 [in Russian].

3. Butenko, R.G. (1999). Biology of cells of higher plants in vitro and biotechnology based on them. M.: FBK-PRESS [in Russian].

4. Dubrovna, O.V. & Morhun, B.V. (2009). Wheat Cell Selection for Resistance to Stressful Environmental Factors. Fiziologiya i biokhimiya kult. rastenii, 41(6), pp. 463-475 [in Ukrainian].

5. Zaprometov, M.N. (1993). Phenolic compounds. Distribution, metabolism and function in plants. M.: Nauka [in Russian].

6. Znamenskaja, V.V. (2010). Microcloning in vitro as a method of maintaining sugar beet lines. Jenciklopedija roda Beta: biologija, genetika i selekcija. Novosibirsk [in Russian].

7. Kliachenko, O.L. & Krylovska, S.A. (2012). Getting sugar beet regenerators. Zbirnyk naukovykh prats Podilskoho derzhavnoho ahrarno-tekhnichnoho universytetu, No. 11, pp. 312-315 [in Ukrainian].

8. Kovalev, V.N., Popova, N.V., Kislichenko, V.S., Isakova, T.I., Zhuravel, I.A., Stepanova, S.I., Serbin, A.N., Seraia, L.M. & Kartmazova, L.S. (2003). Workshop on pharmacogenesis. Kharkov: Izd-vo NFaU Zolotye stranicy [in Russian].

9. Mironenko, N.V. (2011). UV-spectrophotometric determination of triterpene saponins - derivatives of oleanolic acid. Himija rastitel'nogo syr'ja, No. 3, pp. 153-157 [in Russian].

10. Redko, V.I., Roik, M.V., Nediak, T.M., Bekh, N.S. & Slutska, N.P. (2008). Slowly growing in vitro collection of sugar and fodder beet as a method of preserving their genetic diversity. Zbirnyk naukovykh prats ITsB UAAN, Iss. 10, pp.231-234 [in Ukrainian].

11. Roik, M.V. & Korneieva, M.O. (2010). Selection of sugar beet. Special selection of field crops. Bila Tserkva: BTs NAU, 276-314 [in Ukrainian].

12. Charles, S. B., Imin, N. & Djordjevic M.A. (2010). Flavonoids: new roles for old molecules. J. of Integrative Plant Biol., 52 (1), pp. 98-111. https://doi.org/10.1111/j.1744-7909.2010.00905.x

13. Handbook of Food Analytical Chemistry: Pigments, Colorants, Flavors, Texture, and Bioactive Food Components. Ed. by E.Wrolstad. (2005). Pigments and colorants (F. 4). Publisher: Wiley, John & Sons, Incorporated.

14. Kuruppusamy, S. (2009). A review on trends in production of secondary metabolites from higer plants de in vitro tissue, organ and cell cultures. Med. Plants Res., 3(13), pp. 1222-1239.

15. Parry, M.A.J., Reynolds, M.P., Salvucci, M.E., Raines, C., Andralojc, P.J., Xin-Guang Zhu, Price, G.D., Condon, A.G. & Furbank, R.T. (2011). Raising yield potential of wheat. II Encreasing photosyntetic capasity and efficiency. J. Exp. Bot., 62(2), pp. 453-467. https://doi.org/10.1093/jxb/erq304

16. Saad, E. & Raja, A. (2003). In vitro micropropagation of spinach beet (Beta vulgaris L.) under effect of saltstress. Amer. Soc. Plant. Biol. (ASPB), 34(4), p. 503.

17. Santelia, D., Henrichs, S., Vincenzetti, V., Sauer, M., Bigler, L., Klein, M., Bailly, A., Lee, Y., Friml, J., Geisler, M. & Martinoia, E. (2008). Flavonoids redirect PIN-mediated polar auxin fluxes during root gravitropic responses. The J. of Biological Chemistry, 283 (45), pp. 31218-31226. https://doi.org/10.1074/jbc.M710122200

18. Stitt, M., Garhardt, R., Vurzel, B. & Heldt, H. (1983). A role for fructose 2,6-bisphosphate in the regulation of sucrose synthesis in spinach leaves. Plant Physiol., 72, pp. 1139-1143. https://doi.org/10.1104/pp.72.4.1139