The polymorphism of DNA regions, flanked by inverted repeats LTR retrotransposons, in genetically modified wheat plants obtained by Agrobacterium-mediated transformation in vitro comprising ornithine aminotransferase gene has been analyzed. The most effective was the use of primer to the retrotransposon Sukkula, where the spectrum of DNA amplification products revealed four new amplicons in nine plants studied. The findings suggest that it is the insertion of foreign DNA capable of inducing transposition of retrotransposons Sukkula/Nikita and Wham/Sabrina, because in control plants derived from in vitro culture their activity has not been established.
Keywords: Triticum aestivum L., Agrobacterium-mediated transformation, retrotransposons, IRAP-PCR
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