Fiziol. rast. genet. 2016, vol. 48, no. 5, 416-423, doi:

Comparative research of water deficit influence on indexes of water mode and parameters of induction of chlorophyll fluorescence of cotton leaves

Akhmedzhanov I.G., Lukyanova S.V., Agishev V.S., Nabiev S.M., Khotamov M.M., Tonkikh A.K., Usmanov R.M.

  • M. Ulugbek National University of UzbekistanĀ  VUZgorodok, Tashkent, 100174, Uzbekistan

By the methods of seeds germination in the different concentrations of saccharose, measuring of water potential and induction of chlorophyll fluorescence of leaves in field conditions comparative research of influence of water deficit on drought tolerance of cotton varieties was conducted. It is shown that in the conditions of insufficient water supply indices of germination of seeds, water potential and parameters of induction of chlorophyll fluorescence of leaves of all varieties decreased, however the degree of change of indices differs among varieties. In accordance with the obtained data, all varieties allocated to three groups: drought-resisting, with the lowered resistance to a drought and non-resistant to the drought. Accordance of changes of induction curves of chlorophyll fluorescence of leaves and indices of the water status of cotton plants was revealed, that specifies on perspective of the use of the method of induction of chlorophyll fluorescence for diagnostics of drought tolerance of cotton plant genotypes.

Keywords: Gossypium hirsutum L., cotton, drought tolerance, water potential, induction of chlorophyll fluorescence

Fiziol. rast. genet.
2016, vol. 48, no. 5, 416-423

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1. Ahmedzhanov, I.G., Agishev, V.S., Dzholdasova, K.B. & Tashmuhamedov, B.A. (2013). The use of a portable fluorometer to study the effect of water deficiency on the characteristics of delayed fluorescence of cotton leaves. Doklad AN RUz., 3, pp. 58-60 [in Russian].

2. Veselovskiy, V.A. & Veselova, T.V. (1990). Luminescence plants. Theoretical and practical aspects. Moskva: Nauka [in Russian].

3. Doang, Hoang Zhang & Tohtar, V.K. (2011). Investigation of drought tolerance of promising species for introduction of Momordica charantia and Momordica balsamina (Cucurbitaceae). Nauchnyie vedomosti BGNIU., Estestv. nauki, Iss. 15, No. 9 (104), pp. 43-47 [in Russian].

4. Karymova, I.S. (2009). The effect of prolonged soil drought on the physiological processes in various varieties and cotton lines (Unpublished candidate thesis). Dushanbe [in Russian].

5. Korneev, D.Yu. (2002). Informational possibilities of the method of induction of chlorophyll fluorescence. Kiev: Altpres [in Russian].

6. Korneev, D.Yu., Nizhnik, T.P., Grigoryuk, I.A. & Kochubey, S.M. (2002). Fluorescence induction of potato leaf chlorophyll under water deficiency conditions. Fiziologiya i biohimiya kult. rasteniy, 34, No. 1, pp. 67-78 [in Russian].

7. Kushnyrenko, M.D., Goncharova, E.A. & Bondar, E.M. (1970). Methods for studying water metabolism and drought tolerance of fruit plants. Kishinev: Shtiintsa [in Russian].

8. Pikulenko, M.M. & Bulyichev, A.A. (2007). Using the parameters of fluorescence and the generation of electrical potentials in the membranes of plant cells to assess the state of biological objects. Byul. Mosk. o-va ispyit. prirody. Biologiya, 112 (1), pp. 80-84 [in Russian].

9. Tretyakov, N.N., Karnauhova, T.V. & Panichkin, L.A. (1990). Practical work of plant physiology. M.: Agropromyzdat [in Russian].

10. Rekomendatsii NITs MKVK po proektu IUVR-Fergana (Uzbekistan). The need of main crops for irrigation water in phases of development. Tashkent [in Russian].

11. Shadchina, T.M. & Pryadkina, G.A. (2006). The effect of soil salinization and nitrogen nutrition deficiency on the activity of the violaxanthine cycle and non-photochemical quenching of chlorophyll fluorescence in wheat leaves. Fiziologiya i biohimiya kult. rasteniy, 38, No. 3, pp. 214-221 [in Russian].

12. Ackerson, R.C., Krieg, D.R., Miller, T.D. & Zaetman, R.E. (1977). Water relations of field grown cotton and sorghum: Temporal and diurnal changes in leaf water, osmotic, and turgor potentials. Crop Sci., 17, pp. 76-80.

13. Lichtenthaler, H.K. (1992). The Kautsky effect: 60 years of chlorophyll fluorescence induction kinetics. Photosynthetica, 27, No. 1-2, pp. 45-55.

14. Loka, D.A., Oosterhuis, D.M. & Ritchie, G.L. (Eds.). (2011). Water deficit stress in cotton. Stress physiology in cotton. Referens book series . The cotton Foundation. Cordova, Tennessee, USA, No. 7, pp. 37-72.

15. Maxwell, K. & Johnson, G.N. (2000). Chlorophyll fluorescence - a practical guide. Journal of Experimental Botany, 51, No. 345, pp. 659-668.

16. Nyachiro, J.M., Briggs, K.G., Hoddinott, J. & Johnson-Flanagan, A.M. (2001). Chlorophyll content, chlorophyll fluorescence and water deficit in spring wheat. Cereal Research Communications, 29, No. 1-2, pp. 135-142.

17. Posudin, Yu. & Bogdasheva, O. (2010). Fluorescence analysis of green pea Pisum sativum during development and under external factors. Sci. Bull. of NAU, No. 5, pp. 21-28.

18. Posudin, Yu.I., Godlevska, O.O., Zaloilo, I.A. & Kozhem'yako, Ya.V. (2010). Application of portable fluorometer for estimation of plant tolerance to abiotic factors. Int. Agrophysics., 24, No. 4, pp. 363-368.

19. Ronacek, K. & Bartak, M. (1999). Technique of the modulated chlorophyll fluorescence: basic concepts, useful parameters, and some applications. Photosynthetica, 37, No. 3, pp. 339-363.

20. Zlatev, Z.S. & Yordanov, I.T. (2004). Effects of soil drought on photosynthesis and chlorophyll fluorescence in bean plants. Bulg. J. Plant Physiol., 30, pp. 3-18.