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ISSN 2308-7099, ISSN 2786-6874
Физиология растений и генетика
 
 
Физиология растений и генетика 2016, том 48, № 2, 158-166, doi: https://doi.org/10.15407/frg2016.02.158

Оксид азота и пероксид водорода как сигнальные посредники при индуцировании теплоустойчивости проростков пшеницы экзогенными жасмоновой и салициловой кислотами

Карпец Ю.В.1, Колупаев Ю.Е.1, Косаковская И.В.2

  1. Харьковский национальный аграрный университет им. В.В. Докучаева 62483 Харьков, п/о «Коммунист-1»
  2. Институт ботаники им. Н.Г. Холодного Национальной академии наук Украины 01601 Киев, ул. Терещенковская, 2

Исследовано участие оксида азота (NO) и пероксида водорода в индуцировании теплоустойчивости проростков пшеницы (Triticum aestivum L.) действием экзогенных стрессовых фитогормонов — жасмоновой (ЖАК) и салициловой (СК) кислот. Установлено, что 24-часовая обработка проростков ЖАК и СК в концентрациях 1 и 10 мкМ повышает их выживаемость после действия потенциально летального теплового стресса (46 оС, 10 мин). Под влиянием обоих фитогормонов в корнях проростков транзиторно несколько повышалось содержание пероксида водорода с максимумом через 30 мин после начала обработки. Содержание оксида азота под влия­нием ЖАК и СК увеличивалось более существенно и наблюдалось в течение 2 ч с момента начала обработки проростков. Предобработка антиоксидантом диметилтиомочевиной (ДМТМ) нивелировала эффект повышения содержа­ния NO в корнях, вызываемый ЖАК и СК. При предварительном воздействии на проростки ДМТМ, как и при их предобработке скавенжером оксида азота РТIО (2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide) и ингибитором NO-синтазы L-NAME (NG-nitro-L-arginine methyl ester), заметно угне­талось развитие теплоустойчивости, индуцируемое действием ЖАК и СК. Сде­лано заключение о роли NO и пероксида водорода в процессах ЖАК- и СК-индуцированного развития теплоустойчивости проростков пшеницы.

Ключевые слова: Triticum aestivum L., жасмоновая кислота, салициловая кислота, оксид азота, пероксид водорода, трансдукция сигналов, теплоустойчивость

Физиология растений и генетика
2016, том 48, № 2, 158-166

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