The creation of new drugs based on extracts of the «hairy» roots of rare medicinal plants used in traditional medicine can help solve three important problems of biotechnology at once: the search for natural producers of biologically active compounds, the search for new products, and the possibility of intensification of production. Therefore, the aim of this work was to obtain a complex of bioflavonoids in the form of a dry extract of the «hairy» roots of Artemisia tilesii Ledeb. followed by an assessment of its biosafety. For this, a line of «hairy» roots of Tilesius’ wormwood, which has fast growth and sensitivity to methyl jasmonate as an elicitor, that was shown earlier, was chosen. Two-stage cultivation with methyl jasmonate at a concentration of 100 mM revealed the possibility of increasing the content of flavonoids by 3.3 times (up to 27.56±2.52 mg RE/g of FW), which is important for the intensification of the process. The content of dry matter in 1 g of roots obtained after such cultivation was 0.15 g or 14.8 %. From 1 g of lyophilized roots, 270 mg of dry ethanolic extract was obtained with a flavonoid content of 95.53 mg RE/g of dry extract, i.e. 9.56 % of its dry weight. Thus, 4 g of dry extract with a total flavonoid content of 382.12 mg RE can be obtained from 100 g of «hairy» roots. The results of determining the biosafety of the flavonoid-containing composition using the in vitro culture of Lemna minor L. showed that the duckweed leaves number and mass increase was even greater than in the control samples (the number of leaves was 26 % greater and their mass increase was by 35.50 %) and in samples cultivated with flavonoid standards (fisetin, kaempferol, epicatechin, and quercetin for comparison). Such results indicate the non-toxicity and biosafety of the preparation for plants. In addition to non-toxicity, the obtained complex of compounds has been shown to have a growth-stimulating effect. Therefore, the proposed method of obtaining a flavonoid-containing complex based on the «hairy» roots of A. tilesii can be used as a basis for the development of new biosafe preparations of valuable bioactive compounds.
Keywords: Artemisia tilesii Ledeb., flavonoids, biosafety, non-toxicity
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