The proline dehydrogenase (ProDH) gene associated with proline catabolism is of practical importance for genetic engineering, as partial inhibition of its expression can lead to an increase in the content of free proline and, as a result, the level of plant tolerance to abiotic stresses, in particular drought. The aim of our work was to carry out Agrobacterium-mediated transformation of morphogenic calli of new winter bread wheat promising genotypes and obtain genetically modified plants with partial suppression of the proline dehydrogenase gene. The relatively greater efficiency of using the AGL0 strain for obtaining transgenic plants of various genotypes of winter wheat with partial suppression of the proline dehydrogenase gene in culture in vitro was shown. The frequency of the Arabidopsis ProDH target gene sequences insertion when using the LBA4404 strain in the studied genotypes was 0.7—1.7 %, and when using the AGL0 strain — 1.0—2.0 %. The transgenic status of the obtained regenerants was confirmed by PCR analysis. Reverse transcriptase PCR (RT-PCR) using total RNA confirmed the expression of the introduced Arabidopsis proline dehydrogenase gene at the transcription level in transgenic wheat plants obtained by Agrobacterium-mediated transformation. It was established that plants with reduced activity of proline dehydrogenase are characterized by a significantly higher content of free L-proline compared to the non-transgenic control.
Keywords: Triticum aestivum L., Agrobacterium-mediated transformation, callus cultures, proline dehydrogenase gene
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