Fungicide Sedaxane (mix of trans- and cis-isomers of N-[2-(1,1'-bicyclopropyl)-2-yl-phenyl]-3-(difluoromethyl)-1-methyl-1-H-pyrazole-4-carboxamide), relating to inhibitors of succinate dehydrogenase, causes the wide spectrum of physiological reactions related to the shifts of hormonal balance and to the change of expression of large amount of genes in plants. Its influence on the resistance of corn (Zea mays L.) seedlings of hybrids Ariosso and Rotango to the salt stress (germination of seeds on 100 mM NaCl solution) have been investigated. Hybrid Rotango differed by higher salt resistance in comparison with the hybrid Ariosso that was revealed in smaller oppression of growth of shoots and roots under conditions of salt stress. Priming of seeds with Sedaxane in concentration of 0.1 mg/ml significantly softened the negative impact of salt stress on the linear growth and accumulation of seedlings biomass. At the same time the treatment with Sedaxane increased the growth of both shoots and roots in seedlings of hybrid Ariosso under the salt stress, and only shoots in hybrid Rotango. Higher value of superoxide dismutase (SOD) and guaiacol peroxidase activity in shoots under stress conditions were characteristic to hybrid Rotango in comparison with those for the hybrid Ariosso. Under the influence of Sedaxane at the impact of salt stress SOD activity increased in the seedlings of hybrid Ariosso. Activity of other antioxidant enzymes (catalase and guaiacol peroxidase) in variants with priming of seeds with Sedaxane in both hybrids almost did not change. Treatment of seeds of both hybrids with Sedaxane significantly increased the accumulation of proline and caused the tendency to increase in content of carbohydrates and anthocyans under the action of NaCl. Sedaxane also eliminated the increase in content of hydrogen peroxide affected by the salt stress in shoots of seedlings of both genotypes. It was concluded that the intensifying of accumulation of low-molecular antioxidants and osmolytes under the influence of Sedaxane can be one of the causes of increase in the salt resistance of corn.
Keywords: Zea mays L., Sedaxane, salt stress, resistance, reactive oxygen species, antioxidative system, osmoprotective system
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1. Zeun, R., Scalliet, G. & Oostendorp, M. (2013). Biological activity of sedaxane - a novel broad-spectrum fungicide for seed treatment. Pest. Manag. Sci., 69, pp. 527-534. https://doi.org/10.1002/ps.3405
2. Rheinheimer, J. (2012). Succinate dehydrogenase inhibitors. Modern Crop Protection Compounds. Wiley-VCH, Weinheim, Germany, 2, pp. 627-639.
3. Jeschke, P. (2016). Progress of modern agricultural chemistry and future prospects. Pest. Manag. Sci., 72, pp. 433-455. https://doi.org/10.1002/ps.4190
4. Huang, S. & Millar, A.H. (2013). Succinate dehydrogenase: the complex roles of a simple enzyme. Curr. Opin. Plant Biol., 16(3), pp. 344-349. https://doi.org/10.1016/j.pbi.2013.02.007
5. Gleason, C., Huang, S., Thatcher, L., Foley, R.C., Anderson, C.R., Carroll, A.J., Millar, A.H., & Singh, K.B. (2011). Mitochondrial complex II has a key role in mitochondrial-derived reactive oxygen species influence on plant stress gene regulation and defense. Proc. Natl. Acad. Sci. USA, 108, pp. 10768-10773. https://doi.org/10.1073/pnas.1016060108
6. Kolupaev, Yu.E., Karpets, Yu.V., Yastreb, T.O. & Firsova, E.N. (2017). Protective effect of inhibitors of succinate dehydrogenase on wheat seedlings during osmotic stress. Appl. Biochem. Microbiol., 53(3), pp. 353-358. https://doi.org/10.1134/S0003683817030097
7. Ajigboye, O.O., Lu, C., Murchie, E.H., Schlatter, C., Swart, G. & Ray, R.V. (2017). Altered gene expression by sedaxane increases PSII efficiency, photosynthesis and growth and improves tolerance to drought in wheat seedlings. Pestic. Biochem. Physiol., 137, pp. 49-61. https://doi.org/10.1016/j.pestbp.2016.09.008
8. Dal Cortivo, C., Conselvan, G.B., Carletti, P., Barion, G., Sella, L. & Vamerali, T. (2017). Biostimulant effects of seed-applied sedaxane fungicide: morphological and physiological changes in maize seedlings. Front. Plant Sci., 8: 2072. https://doi.org/10.3389/fpls.2017.02072
9. Munns, R. (2002). Comparative physiology of salt and water stress. Plant Cell Environ., 25, pp. 239-250. https://doi.org/10.1046/j.0016-8025.2001.00808.x
10. Isayenkov, S.V. (2012). Physiological and molecular aspects of salt stress in plants. Cytol. Genet., 46, pp. 302-318. https://doi.org/10.3103/S0095452712050040
11. Rozentsvet, O.A., Nesterov, V.N. & Bogdanova, E.S. (2017). Structural, physiological, and biochemical aspects of salinity tolerance of halophytes. Rus. J. Plant Physiol., 64(4), pp. 464-477. https://doi.org/10.1134/S1021443717040112
12. Matysik, J., Alia, B., Bhalu, B. & Mohanty, P. (2002). Molecular mechanism of quenching of reactive oxygen species by proline under stress in plant. Curr. Sci., 82, pp. 525-532.
13. Kolupaev, Yu.E., Firsova, K.M., Shvidenko, M.V. & Yastreb, T.O. (2018). Hydrogen sulfide donor influence on state of antioxidant system of wheat seedlings under osmotic stress. Fiziol. rast. genet., 50, No. 1, pp. 29-38. https://doi.org/10.15407/frg2018.01.029
14. Nogues, S. & Bakerm, N.R. (2000). Effects of drought on photosynthesis in Mediterranean plants grown under UV-B radiation. J. Exp. Bot., 51, pp. 1309-1317. https://doi.org/10.1093/jxb/51.348.1309
15. Bates, L.S., Walden, R.P. & Tear, G.D. (1973). Rapid deter-mination of free proline for water stress studies. Plant Soil, 39(1), pp. 205-210. https://doi.org/10.1007/BF00018060
16. Zhao, K., Fan, H., Zhou, S. & Song, J. (2003). Study on the salt and drought tolerance of Suaeda salsa and Kalanchoe claigremontiana under iso-osmotic salt and water stress. Plant Sci., 165(4), pp. 837-844. https://doi.org/10.1016/S0168-9452(03)00282-6
17. Sagisaka, S. (1976). The occurrence of peroxide in a perennial plant, Populus gelrica. Plant Physiol., 57, pp. 308-309. https://doi.org/10.1104/pp.57.2.308
18. Szabados, L. & Savoure, A. (2010). Proline: a multifunctional amino acid. Trends Plant Sci., 15(2), pp. 89-97. https://doi.org/10.1016/j.tplants.2009.11.009
19. Liang, X., Zhang, L., Natarajan, S.K. & Becker, D.F. (2013). Proline mechanisms of stress survival. Antioxid. Redox Signal., 19, pp. 998-1011. https://doi.org/10.1089/ars.2012.5074
20. Signorelli, S., Coitino, E.L., Borsani, O. & Monza, J. (2014). Molecular mechanisms for the reaction between OH radicals and proline: insights on the role as reactive oxygen species scavenger in plant stress. J. Phys. Chem., 118(1), pp. 37-47. https://doi.org/10.1021/jp407773u
21. Khlestkina, E.K. (2013). The adaptive role of flavonoids: emphasis on cereals. Cereal Res. Commun., 41, pp. 185-198. https://doi.org/10.1556/CRC.2013.0004
22. Kolupaev, Yu.E. (2016). Plant cell antioxidants and their role in ROS signaling and plant resistance. Uspekhi Sovrem. Biologii, 136(2), pp. 181-198 [in Russian].