Fiziol. rast. genet. 2019, vol. 51, no. 4, 315-323, doi:

Cell selection with heavy metal ions for obtaining salt tolerant plant cell cultures

Sergeeva L.E., Mykhalska S.I.

  • Institute of Plant Physiology and Genetics, National Academy of Sciences of Ukraine 31/17 Vasylkivska St., Kyiv, 03022, Ukraine

The cell selection with heavy metal ions was proposed for obtaining salt tolerant plant cell lines. On selective media with the addition of Ba2+ cations resistant soybean and wheat cell variants were obtained. Doses of Ba2+ cations were lethal for wild type cell cultures. The selected clones were tested under stress pressure of various agents: Ba2+ ions, sea water salts, sodium sulfate. Resistant cells sustained any type of lethal stress. Calli retained salt tolerance after cultivation on salt-free medium during several passages. During cultivation under salinity cells demonstrated low K+/Na+ ratios and high free proline accumulation. The Ba2+ provoked opposite cell reactions. It is assumed that combined stress resistance is concerned with realization of different protection mechanisms.

Keywords: soybean, wheat, cell selection, Ba2+ cations, salt tolerance

Fiziol. rast. genet.
2019, vol. 51, no. 4, 315-323

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1. Sergeeva, L.E. (2013). Cell selection with heavy metal ions for obtaining plant genotypes with combined resistance to abiotic stresses. Kiev: Logos [in Russian].

2. Tu, S.-I., Nungesser, E. & Brauer, D. (1989). Characterization of the effect of divalent cations on the coupled activities of the H+-ATPase in tonoplast vesicles. Plant Physiol., 90, pp.1636-1643.

3. Miller, R.J., Dumford, S.W., Koeppe, D.E. & Hanson, J.B. (1970). Divalent cation stimulation of substrate oxidation by corn mitochondria. Plant Physiol., 45, pp. 649-653.

4. Hasenstein, K.H., Evans, M.L., Stinemetz, C.L., Moore, R., Fondrren, M., Koon, C., Higby, M. & Smucker, A.J.M. (1988). Comparative effectiveness of metal ions in inducing curvature in primary roots of Zea mays. Plant Physiol., 86, pp. 885-889.

5. Fan, L.M., Wu, W.-H. & Yang, Y.-Y. (1999). Identification and characterization the inward K+ channel in the plasma membrane Brassica pollen protoplasts. Plant Cell Physiol., 40 (8), pp. 859-865.

6. Rubio, F., Nieves-Cordones, M., Aleman, F. & Martinez, V. (2008). Relative contribution of AtHAK5 and AtHAK1 to K+ uptake in the high affinity range of concentrations. Physiol. Plant., 134, pp. 598-608.

7. Wang, D.-M., Zhang, J.-L. & Flowers, T.J. (2007). Low affinity Na+ uptake in the halophyte Suaeda maritima. Plant Physiol., 145, pp. 559-571.

8. Hasegawa, P.M., Bressan, R.A., Zhu, J.K. & Bohnert, H.J. (2000). Plant cellular and molecular responses to high salinity. Annu. Rev. Plant Physiol. Plant Mol. Biol., 51, pp. 463-499.

9. Razavizadeh, R. & Ehsanpour, A.A. (2009). Effects of salt stress on proline content, expression of delta-1-pyrroline-5-carboxilate synthetase, activities of catalase and ascorbate peroxidase in transgenic tobacco plants. Biol. Lett., 46 (2), pp. 63-75. https://

10. Gamborg, J.L., Miller, R.A. & Ojima, K. (1968). Nutrient requirement of suspension cultures of soybean roots. Exp. Cell Res., 509, pp. 151-158.

11. Conner, A.J. & Meredith, C.P. (1985). Large scale selection of aluminum-resistant mutants from plant cell culture: expression and inheritance in seedlings. Theor. Appl. Genet., 71, pp. 159-165.

12. Mykhalska, S.I., Sergeeva, L.E. & Tishchenko, E.N. (2010). Cytogenetical analysis of soybean tungsten-resistant cell line. Fiziol. biokhim. kult. rastenii, 42, No. 2, pp. 125-131 [in Russian].

13. Maliga, P. (1984). Isolation and characterization of mutants in plant cell culture. Ann. Rev. Plant Physiol., 35, pp. 519-542.

14. Kant, S., Kant, P., Rahen, E. & Barak, S. (2006). Evidence that differential gene expression between the halophyte Thellungiella halophila and Arabidopsis thaliana is responsible for higher levels of the compatible osmolyte proline and tight control of Na+ uptake in T. halophila. Plant Cell Environ., 29, pp. 1220-1234.

15. Tester, M. & Davenport, R. (2003). Na+ and Na+ transport in higher plants. Ann. Bot., 91, pp. 503-527.

16. Demidchik, V. & Tester, M. (2002). Sodium fluxes through nonselective cation channels in the plasma membrane of protoplasts from Arabidopsis roots. Plant Physiol., 128, pp. 379-387.

17. Arend, M., Stinzing, A., Wind, C., Langer, K., Latz, A., Ache, P., Fromm, J. & Hedrich, R. (2005). Polar-localised popla K+ channel capable to controlling electrical properties of wood-forming cells. Planta, 223, pp. 140-148.

18. Maathuis, F.J.M., Filatov, V., Herzyk, P., Krijer, G.C., Axelsen, K.B., Chen, S., Green, B.J., Madagan, K.L., Sanchez-Fernandez, R. & Forde, B.G. (2003). Transcriptome analysis of root transporters reveals participation of multiple gene families in the response to cation stress. Plant J., 35, pp. 675-692.

19. Volkov, V., Wang, B., Dominy, P.J., Fricke, W. & Amtmann, A. (2004). Thellungiella halophila, a salt-tolerant relative of Arabidopsis thaliana, possesses effective mechanisms to discriminate between potassium and sodium. Plant Cell Environ., 27, pp. 1-14.

20. Amtmann, A., Bohnert, H.J. & Bressan, R.A. (2005). Abiotic stress and plant genome evolution. Search for new models. Plant Physiol., 138, pp. 127-130.

21. Sergeeva, L.E & Bronnikova, L.I. (2017). Cell selection with Ba2+ cations for obtaining salt resistant wheat lines. Fiziol. rast. genet., 49, No. 2, pp. 174-178 [in Russian].

22. Mykhalskaya, S.I. & Sergeeva, L.E. (2007). Free proline content of soybean cell lines cultivated under stress pressure of various agents. Visn. ukr. tov. genet. selec., No. 1-2, pp. 33-38 [in Russian].