The effect of presowing treatment with electromagnetic radiation (EMP) of the millimeter-wavelength range and microconcentrations of epine and 5-aminolevulinic acid (ALA) on the composition of black cumin seed oil was studied. The experiment was carried out with seeds obtained in the harvests of 2014—2016 years. Extraction of oil from black cumin seeds was carried out in Soxhlet apparatus. The qualitative and quantitative compositions of the oil were determined by GC and GC-MS methods. It was found that oil from Nigella sativa grown in Belarus contained myristic, palmitic, stearic, oleic, linoleic, linolenic, arachidic, eicosadienic acids (FA), and thymoquinone, p-cymene, g-terpinene, carvacrol, thymol, yunipen, (+)-isomenthol. The content of unsaturated fatty acids has varied depending on the year of cultivation and the type of presowing treatment from 75.3 to 78.9 % of the total quantity of compounds. Maximum amount, in percentage, was accounted for linoleic acid (56.4—61.9 %), followed by oleic acid (13.4—16.8 %), eicosadienic (2.8—3.6 %) and linolenic acid (0.36—0.68 %). It was established that chemical presowing treatment increased content of thymoquinone and p-cymene in Nigella sativa L. seeds, while the physical one decreased amount of these compounds in relation to control plants. The relation between the content of thymoquinone and the mode of exposure to electromagnetic radiation was found. In order to improve the quality of black cumin oil, it is suggested to use presowing treatment with ALA in 10-6 % concentration which allows to increase thymoquinone content.
Keywords: Nigella sativa L., millimeter-wave electromagnetic radiation, 5-aminolevulinic acid, epin, fatty acids, thymoquinone, p-cymol
Full text and supplemented materialsFree full text: PDF
1. Rud, N.K., Sampiyev, A.M. & Davitavyan, N.A. (2013). The main results of the phytochemical and pharmacological studies of black cumin. Scientific statements. Ser. Medicine. Pharmacy, No. 25 (168), is. 24, pp. 207-212.
2. Bourgou, S., Ksouri, R., Bellila, A., Skandrani, I., Falleh, H. & Marzouk, B. (2008). Phenolic composition and biological activities of Tunisian Nigella sativa L. shoots and roots. C. R. Biol., No. 331, pp. 48-55. https://doi.org/10.1016/j.crvi.2007.11.001
3. Cheikh-Rouhou, S., Besbes, S., Lognay, G., Blecker, C., Deroanne, C. & Attia, H. (2008). Sterol composition of black cumin (Nigella sativa L.) and Aleppo pine (Pinus halepensis Mill) seed oils. J. Food. Compos. Anal., No. 21(2), pp. 162-168.
4. Mashirova, S. Yu. & Oryol, T.V. (2012). Study of the lipid component composition of seeds of Nigella sativa and Nigella damascene. Scientific Reports. Ser. Medicine. Pharmacy, No. 4 (123), is. 17, pp. 223-227.
5. Nickavar, B., Mojab, F., Javidnia, K. & Amoli, M.A. (2003). Chemical composition of the fixed and volatile oils of Nigella sativa L. from Iran. J. Zeitschrift fur Naturforschung, No. 58(9-10), pp. 629-631. https://doi.org/10.1515/znc-2003-9-1004
6. Guliyeva, M.G., Mamedbeyli, A.I., Medzhidova, S.R., Zeynalova, E.I. & Figarova, N.A. (2014). Efficiency of using Black cumin oil to treat herpetic keratitis. Ophthalmology, No. 1 (14), pp. 64-67.
7. Tambiev, A.Kh. (2014). The influence of electromagnetic waves of the millimeter range of low intensity on photosynthesizing organisms: the development of the direction (review). J. Biomedical Radioelectronics, No. 6. pp. 4-18.
8. Chailakhyan, R.K., Yusupov, V.I., Gorskaya, Yu.F., Kuralesova, A.I., Gerasimov, Yu.V., Sviridov, A.P., Tambiev, A.Kh., Vorobieva, N.N., Grosheva, A.G., Shishkova, V.V., Moskvina, I.L. & Bagratashvili, V.N. (2016). Effect of acoustic pulses and EHF radiation on multipotent marrow stromal cell in tissue engineering constructions. J. of innovative optical health sciences, 10, No. 1, pp. 1650036-1-1650036-9 DOI.
9. Tambiev, A.Kh. & Lukyanov, A.A. (2011). Possibilities of diagnosing the effect of EHF and microwave radiation on cyanobacteria, microalgae, and actinomycetes (review). J. Biomedical Radioelectronics, No. 2, pp. 39-53.
10. Yaronskaya, E.B., Averina, N.G. & Kissel, M.A. (2012). Environmentally safe plant growth regulators based on 5-aminolevulinic acid. Proceedings of the Belarusian State University, 7, pp. 127-134.
11. Averina, N.G. & Yaronskaya, E.B. (2012). Biosynthesis of tetrapyrrole in plants. Minsk: Belarus science, 414 p.
12. Shysh, S.N., Shutova, A.G. & Mazetz, J.E. (2013). Comparative estimation of the influence of 5-aminolevulinic acid and epin on the growth process of Calendula officinalis L. Proceedings of the Belarusian State University, 8, Pt 2, pp. 125-129.
13. Averina, N.G. (2016). The role of 5-aminolevulinic acid in the life of plants. Minsk: Izd. Center of BSU, Pt 2, pp. 21-24.
14. Prischepchik, Yu.V. & Averina, N.G. (2016). Influence of biopriming of flax seeds with 5-aminolevulinic acid on germination energy and germination. Minsk: Izdat. Tsentr, pp. 76-79.
15. Gal, L.N. (2014). Physical principles of the functioning of the matter of a living organism. SPb.: Publishing House Polytechnic. University, 400 p.
16. Karpovich, V.A. & Rodionov, V.N. Patent of the Republic of Belarus No. 5580. Method of presowing treatment of seeds of vegetable or grain crops. Issued. 06.23.2003.
17. GOST R 51486-99. Vegetable oils and animal fats. Preparation of fatty acid methyl esters from triglycerides by transesterification with a methanolic solution of sodium methylate [Text]. Enter 2001-01-01. M.: Standardinform, 5th reprint 2008.
18. Frank, D., Pat, S. & Vickers, A.K. (2005). Column selection for the analysis of fatty acid methyl esters. Agilent technologies Inc., USA, pp. 1-12.
19. Aftab, A.K., Mahesar, S.A., Khaskheli, A.R., Sherazi, S.T.H., Sofia, Q. & Zakia, K. (2014). Gas chromatographic coupled mass spectroscopic study of fatty acids composition of Nigella sativa L. (KALONJI) oil commercially available in Pakistan. Intern. Food Res. J., No. 21(4), pp. 1533-1537.
20. Perova, V.G., Dmitriev, L.B., Belopukhov, S.L., Lukomets, V.M. & Dmitrieva, V.L. (2015). Changes in the chemical composition of oil flax lipids under the influence of herbicides - acetyl coenzyme-A-carboxylase inhibitors. News TSCA, Is. 6, pp. 58-66.
21. Fuksman, I.L. (1999). The influence of natural and anthropogenic factors on the metabolism of substances of secondary origin in woody plants. Abstract of dissertation for the degree of Doctor of Biological Sciences. SPb. 43 p.
22. Skakovskiy, E.D., Tychinskaya, L.Yu., Shysh, S.N., Shutova, A.G. & Lamotkin, S.A. (2015). NMR analysis of chloroform extracts of black cumin seeds. Proceedings of BSTU, Chemistry Series, Substance Technology, and Biotechnology, No. 4 (177), pp. 234-239.
23. Fedotov, S.V. (2015). Essential oils of monard species Monarda fistulosa L., Monarda didyma L., Monarda citriodora Cervantes ex Lag., Their chemotypes and biological activity. Collection of scientific papers of the GNSS, 141, pp. 131-147.