The proline level and sucrose/monosaccharides ratio in R1 tobacco plants, obtained from Cd-resistant cell lines were investigated. Those plants challenged the lethal for wild type plants water stress (0,3 M mannitol). The proline level increased, sucrose content and carbohydrate ratio decreased in plants on 5 day of stress pressure. These data reflects the proline key role in plants, cultivated under permanent stress conditions. But the proline content reduced and sucrose content and carbohydrate ratio raised in adequate proportions, if water stress increased (0,6 M mannitol). The osmolytes developed common protective role under those conditions. Stress tolerance was detected for R1 plants during recovery period too.
Keywords: tobacco, cell selection, cadmium cations, water deficit, tolerance, proline, sucrose, monosaccharides
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